外源ABA对木薯叶片β-胡萝卜素合成通路相关基因表达的影响

发布时间：2019-04-28 08:18

【摘要】：探究不同浓度外源ABA对木薯叶片β-胡萝卜素合成途径相关基因的影响。用HPLC测定β-胡萝卜素含量的变化,用q RT-PCR的方法研究木薯胡萝卜素合成途径相关基因的表达水平。喷施20 mg/L外源ABA,木薯叶片β-胡萝卜素含量升高,叶片中涉及类胡萝卜素合成的基因LYCB上调表达,而类胡萝卜素裂解的CCD1基因下调表达。同时,核心酶PSY1与PSY2基因的下调表达与类胡萝卜素含量的变化无关联。而分别采用10 mg/L与80 mg/L浓度喷施后β-胡萝卜素含量均降低,涉及β-胡萝卜素合成通路类胡萝卜素合成相关基因表达水平下降,而经10 mg/L的外源ABA处理催化类胡萝卜素裂解的CCD1基因上调表达。研究表明太低和太高浓度的外源ABA处理木薯叶片导致β-胡萝卜素积累降低,但外源ABA浓度为20 mg/L时能显著提高叶片β-胡萝卜素含量。
[Abstract]:To investigate the effects of exogenous ABA at different concentrations on 尾-carotene synthesis pathway genes in cassava leaves. The changes of 尾-carotene content were determined by HPLC and the expression level of cassava carotene synthesis pathway-related genes was studied by Q-RT-PCR. The content of 尾-carotene in leaves of cassava treated with 20 mg/L exogenous ABA, increased, and the expression of gene LYCB involved in carotenoid synthesis was up-regulated in leaves, while the expression of CCD1 gene was down-regulated by carotenoid cleavage. At the same time, the down-regulated expression of core enzyme PSY1 and PSY2 gene was not related to the change of carotenoid content. The content of 尾-carotene decreased after spraying with 10 mg/L and 80 mg/L respectively, which involved the decrease of gene expression level related to carotenoid synthesis in 尾-carotene synthesis pathway. The expression of CCD1 gene was up-regulated by the treatment of 10 mg/L exogenous ABA to catalyze carotenoid cleavage. The results showed that the accumulation of 尾-carotene was decreased in cassava leaves treated with too low and too high concentration of exogenous ABA, but the content of 尾-carotene in cassava leaves was significantly increased when the concentration of exogenous ABA was 20 mg/L.
【作者单位】：海南大学农学院中国热带农业科学院热带作物品种资源研究所农业部木薯种质资源保护与利用重点实验室
【基金】：国家自然科学基金面上项目(No.31271776) 海南省高层次创新创业人才基金(No.2012-2016)
【分类号】：S533

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