大豆脯氨酸生物合成相关基因Glyma01g24530的功能预测及表达分析

发布时间：2019-05-06 07:41

【摘要】：干旱胁迫下的脯氨酸积累在许多植物中都存在,人们普遍认为脯氨酸含量的提高促进了植物对干旱胁迫的抵抗能力。拟南芥中研究发现,△1-吡咯啉-5-羧酸合成酶基因P5CS1是一种限速酶用来催化脯氨酸生物合成,对脯氨酸非常重要。本研究通过生物信息学及荧光定量PCR对大豆中的P5CS1同源基因Glyma01g24530进行了初步的功能分析和表达模式验证。结果发现:Glyma01g24530具有保守的N端乙酰谷氨酸合成酶激酶结构域和N端乙酰谷氨酸合成酶激酶结构域,系统进化树显示与各植物中P5CS家族成员相似性很高,启动子顺式作用元件序列分析表明该基因存在逆境胁迫、光反应等元件。表达模式分析显示Glyma01g24530可以被干旱、盐诱导表达,并在多数组织中表达。
[Abstract]:The accumulation of proline under drought stress exists in many plants. It is generally believed that the increase of proline content promotes the resistance of plants to drought stress. In Arabidopsis thaliana, 1-pyrrolidine-5-carboxylic acid synthetase gene P5CS1 is a rate-limiting enzyme used to catalyze proline biosynthesis, which is very important for proline. In this study, the functional analysis and expression pattern verification of P5CS1 homologue Glyma01g24530 in soybean were carried out by bioinformatics and fluorescence quantitative PCR (FQ-PCR). The results showed that Glyma01g24530 had conserved N-terminal acetylglutamic acid synthase kinase domain and N-terminal acetylglutamic acid synthase kinase domain. Phylogenetic tree showed high similarity with P5CS family members. Sequence analysis of cis-acting elements of promoter indicated that there were some elements such as stress, light response and so on. Expression pattern analysis showed that Glyma01g24530 could be expressed in most tissues induced by drought and salt.
【作者单位】：东北农业大学大豆研究所/大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室;
【基金】：黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2016005) 抗逆转基因大豆新品种培育(2016ZX08004002)
【分类号】：S565.1

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