盐胁迫诱导盐穗木Rev1、Rev3基因的表达分析
发布时间：2019-05-09 04:31
【摘要】：根据盐穗木盐胁迫下响应的转录组测序结果,参考盐穗木HcRev1、HcRev3基因的ESTs序列设计荧光定量PCR特异性引物,建立检测盐穗木Revs基因相对表达量的荧光定量PCR方法,分析Rev1和Rev3基因在盐穗木不同浓度盐胁迫处理不同时间的转录水平。结果表明,HcRev1、HcRev3基因具有相似的表达模式,在100mmol·L~(-1)NaCl低盐胁迫下表达稳定,在300、500、700 mmol·L~(-1)NaCl胁迫下,随胁迫浓度增高、胁迫时间延长,表达量升高。其中HcRev1在700 mmol·L~(-1)NaCl胁迫14 d后达到峰值,是对照组的4.63倍。HcRev3基因在300mmol·L~(-1)NaCl胁迫14 d时,表达量迅速升高,是对照组的15.55倍,表达差异极显著。研究结果说明HcRev1、HcRev3基因都受盐胁迫诱导表达,提示HcRev1、HcRev3基因虽然表达量存在差异,但在盐胁迫过程中参与了DNA损伤修复。研究有助于阐明Rev1、Rev3基因在DNA损伤修复和植物耐盐性间的调控功能作用。
[Abstract]:According to the sequence analysis of transcription set of response to salt stress of salt panicle wood, the fluorescent quantitative PCR specific primers were designed according to the ESTs sequence of HcRev1,HcRev3 gene in salt panicle wood, and a fluorescence quantitative PCR method was established to detect the relative expression of Revs gene in salt panicle wood. The transcription levels of Rev1 and Rev3 genes in different concentrations of salt stress were analyzed. The results showed that the expression pattern of HcRev1,HcRev3 gene was similar, and it was stable under low salt stress of 100mmol 路L ~ (- 1) NaCl. Under the stress of 300500700 mmol 路L ~ (- 1) NaCl, the expression level increased with the increase of stress concentration and the prolongation of stress time. The expression of HcRev1 reached its peak at 14 days after NaCl stress, which was 4.63 times higher than that of the control group. The expression of HcRev3 gene increased rapidly at 14 days of 300mmol 路L-1 NaCl stress, which was 15.55 times higher than that of the control group, and the difference was very significant. The results showed that the expression of HcRev1,HcRev3 gene was induced by salt stress, suggesting that although the expression of HcRev1,HcRev3 gene was different, it was involved in the repair of DNA damage during salt stress. The study may help to elucidate the regulatory function of Rev1,Rev3 gene between DNA damage repair and salt tolerance in plants.
【作者单位】： 新疆大学生命科学与技术学院;新疆生物资源基因工程重点实验室;
【基金】：新疆重点实验室专项资金资助项目(2014KL001)~~
【分类号】：Q943.2
本文编号：2472474
[Abstract]:According to the sequence analysis of transcription set of response to salt stress of salt panicle wood, the fluorescent quantitative PCR specific primers were designed according to the ESTs sequence of HcRev1,HcRev3 gene in salt panicle wood, and a fluorescence quantitative PCR method was established to detect the relative expression of Revs gene in salt panicle wood. The transcription levels of Rev1 and Rev3 genes in different concentrations of salt stress were analyzed. The results showed that the expression pattern of HcRev1,HcRev3 gene was similar, and it was stable under low salt stress of 100mmol 路L ~ (- 1) NaCl. Under the stress of 300500700 mmol 路L ~ (- 1) NaCl, the expression level increased with the increase of stress concentration and the prolongation of stress time. The expression of HcRev1 reached its peak at 14 days after NaCl stress, which was 4.63 times higher than that of the control group. The expression of HcRev3 gene increased rapidly at 14 days of 300mmol 路L-1 NaCl stress, which was 15.55 times higher than that of the control group, and the difference was very significant. The results showed that the expression of HcRev1,HcRev3 gene was induced by salt stress, suggesting that although the expression of HcRev1,HcRev3 gene was different, it was involved in the repair of DNA damage during salt stress. The study may help to elucidate the regulatory function of Rev1,Rev3 gene between DNA damage repair and salt tolerance in plants.
【作者单位】： 新疆大学生命科学与技术学院;新疆生物资源基因工程重点实验室;
【基金】：新疆重点实验室专项资金资助项目(2014KL001)~~
【分类号】：Q943.2
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