西瓜BADH基因序列分析及其乙烯和茉莉酸甲酯诱导表达特性

发布时间：2019-05-14 14:12

【摘要】：甜菜碱作为植物体内一种重要的渗透调节物,对调节逆境下植物细胞的渗透压起着十分重要的作用,而甜菜碱醛脱氢酶(BADH)在植物甜菜碱合成过程中起了关键的催化作用。本研究中,通过分析西瓜BADH基因的结构和功能,结果表明:ClBADH基因启动子区结构中含有15个外显子和14个内含子,其含有包括乙烯响应元件在内的20多种顺式响应元件;ClBADH基因编码的蛋白含有503个氨基酸,该蛋白分子量约为54.5 kD;蛋白亚细胞定位预测显示其可能定位在细胞质中,具有氧化还原酶活性。通过对系统进化树的分析发现西瓜在同源关系上与同科的黄瓜和甜瓜更近,而与藜科的甜菜的同源关系相对较远。此外,以茉莉酸甲酯和乙烯两种物质模拟逆境信号处理西瓜细胞,发现两种信号物质诱导后,西瓜ClBADH基因的表达量均有上调的趋势,但其上调的速度和幅度却存在明显的差异。本研究为通过逆境信号诱导提高西瓜抗逆性提供参考,也为利用基因工程技术提高西瓜抗逆性提供支持。
[Abstract]:Betaine, as an important osmotic regulator in plants, plays a very important role in regulating the osmotic pressure of plant cells under stress, and betaine aldehyde dehydrogenase (BADH) plays a key catalytic role in the synthesis of plant betaine. In this study, the structure and function of watermelon BADH gene were analyzed. The results showed that there were 15 exons and 14 introns in the promoter region of ClBADH gene, and it contained more than 20 kinds of cis-response elements, including ethylene response elements. The protein encoded by ClBADH gene contains 503 amino acids. The molecular weight of the protein is about 54.5 kD; protein subcellular localization prediction shows that it may be located in the cytoplasm and has redox enzyme activity. Through the analysis of phylogenetic tree, it was found that watermelon was closer to cucumber and muskmelon of the same family in homology relationship, but relatively far from sugar beet of Chenopodiaceae. In addition, methyl jasmonate and ethylene were used to simulate stress signal processing of watermelon cells. It was found that the expression of ClBADH gene in watermelon was up-regulated after induction of the two signal substances, but the speed and amplitude of upregulation were significantly different. This study provides a reference for improving the stress resistance of watermelon by stress signal induction, and also provides support for the use of genetic engineering technology to improve the stress resistance of watermelon.
【作者单位】：海南省热带生物资源可持续利用重点实验室(省部共建国家重点实验室培育基地)海南大学热带农林学院;中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室;
【基金】：海南省自然科学基金项目(20153059) 海南大学中西部计划学科建设项目(ZXBJH-XK008) 中国热带农业科学院基本科研业务费专项基金(1630052016006)共同资助
【分类号】：Q943.2;S651

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