山羊CD36基因表达差异及其与肌内脂肪沉积的关系研究

发布时间：2019-05-20 12:45

【摘要】：为了构建山羊CD36基因的组织表达谱及揭示基因表达与肌内脂肪(IMF)沉积的关系,试验采用荧光定量PCR(q PCR)技术检测CD36基因在山羊不同器官组织和三个发育阶段的不同肌肉组织表达情况,并将基因mRNA表达量与IMF含量进行相关分析。结果表明:CD36基因在山羊心脏、肝脏、脾脏、肺脏、肾脏、脂肪和背最长肌等器官组织中均有表达,其中在脂肪组织中表达量最高,在脾脏中次之,而在心脏中表达量最低。CD36基因在1~3月龄、8~10月龄时表达变化趋势相同,在背最长肌、股二头肌和臂三头肌中呈逐渐上升趋势,而24月龄时则呈相反变化趋势。相关性分析表明,肌肉组织中CD36基因mRNA表达量与IMF含量呈正相关,且在臂三头肌中相关性达到显著水平(P0.05)。说明CD36基因可以作为山羊IMF沉积的候选基因。
[Abstract]:In order to construct the tissue expression profile of goat CD36 gene and reveal the relationship between gene expression and intramuscular fat (IMF) deposition, Fluorescence quantitative PCR (q PCR) (FQ-PCR) was used to detect the expression of CD36 gene in different organs and tissues of goats and different muscle tissues at three developmental stages, and the correlation between mRNA expression and IMF content was analyzed. The results showed that CD36 gene was expressed in goat heart, liver, spleen, lung, kidney, fat and longissimus dorsi muscle, among which the expression was the highest in adipose tissue and the second in spleen. The expression of CD36 gene in the heart was the lowest. The expression trend of CD36 gene was the same at the age of 1 ~ 3 months and 8 脳 10 months old, and increased gradually in the longissimus dorsi muscle, biceps femoris and triceps femoris, but showed the opposite trend at 24 months of age. Correlation analysis showed that the expression of CD36 gene mRNA in muscle tissue was positively correlated with IMF content, and the correlation was significant in triceps muscle (P 0.05). It is suggested that CD36 gene can be used as a candidate gene for goat IMF deposition.
【作者单位】：青藏高原动物遗传资源保护与利用四川省重点实验室;贵州省播州区泮水镇农业服务中心;西南民族大学生命科学与技术学院;
【基金】：四川省教育厅项目(15ZA0385)
【分类号】：S827

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