饲料添加益生菌对凡纳滨对虾肠道菌群、Toll受体及溶菌酶基因表达及抗感染的影响

发布时间：2019-05-22 12:18

【摘要】：以初体重为(7.20±1.38)g的凡纳滨对虾(Litopenaeus vannamei)为研究对象,在室内养殖箱进行3周的养殖实验和2周的哈维氏弧菌(Vibrio harveyi)人工感染实验,其中对照组每日投喂普通商品饲料,实验组每日投喂在普通商品饲料中添加地衣芽孢杆菌(Bacillus licheniformis)1.0×10~8 CFU/m L配制成的实验饲料。目的是研究饲料中添加益生菌对凡纳滨对虾肠道菌群、Toll受体及溶菌酶基因表达量和抗哈维氏弧菌能力的影响。实验结果表明,在饲料中添加地衣芽孢杆菌可显著提高对虾抗哈维氏弧菌感染的能力,其相对免疫保护率为22.22%。与对照组相比,实验组可显著降低对虾肠道内弧菌数量(P0.05)。感染哈维氏弧菌后,实验组溶菌酶(lysozyme,LZM)m RNA的相对表达量在12 h、18 h、24 h、36 h、48 h、72 h均显著高于对照组(P0.05)。实验组感染哈维氏弧菌后在6 h、12 h、18 h、24 h、36 h、48 h、72 h、7 d的Toll受体(Toll receptor)m RNA的相对表达量均显著高于对照组(P0.05)。实验结果提示:饲料中添加地衣芽孢杆菌可有效提高对虾抗哈维氏弧菌感染的能力,这种能力的提高可能是通过降低对虾肠道内的弧菌量,并提高抗病相关基因的表达量实现的。
[Abstract]:The (Litopenaeus vannamei) of Penaeus vannamei, whose initial body weight was (7.20 卤1.38) g, was cultured in an indoor culture box for 3 weeks and Vibrio Haavi (Vibrio harveyi) artificial infection experiment for 2 weeks. The control group was fed with common commercial feed every day, and the experimental group was fed with Bacillus licheniformis (Bacillus licheniformis) 1.0 脳 10 ~ 8 CFU/m / L daily. Objective to study the effect of probiotics on intestinal flora, Toll receptor, lysozyme gene expression and vibrio Haavi resistance in Penaeus vannamei. The results showed that Bacillus licheniformis could significantly improve the ability of resistant to Vibrio Haavi infection in shrimp, and the relative immune protection rate was 22.22%. Compared with the control group, the number of Vibrio in the intestinal tract of Penaeus chinensis was significantly decreased in the experimental group (P 0.05). After infection with Vibrio Haavei, the relative expression of lysozyme (lysozyme,LZM) m RNA) in the experimental group was significantly higher than that in the control group at 12 h, 18 h, 36 h, 48 h and 72 h (P 0.05). The relative expression of Toll receptor (Toll receptor) m RNA in the experimental group was significantly higher than that in the control group at 6 h, 12 h, 18 h, 36 h, 72 h, 7 d after infection (P 0.05). The results suggest that the addition of Bacillus licheniformis in feed can effectively improve the ability of resistant to Vibrio Haavi infection in prawns, and this ability may be improved by reducing the amount of Vibrio in the intestinal tract of Penaeus vannamei. The expression of disease resistance related genes was increased.
【作者单位】： 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所;上海海洋大学水产与生命学院;
【基金】：国家973计划项目(2012CB114405) 农业部引进国际先进农业科学技术计划项目(2016-X56)
【分类号】：S968.22

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