应用抑制性差减杂交技术研究枣缩果病病程相关基因
发布时间：2019-05-24 17:41
【摘要】：【目的】了解细交链孢菌侵染枣果实过程的差异表达基因。【方法】以白熟期‘蜂蜜罐’枣的果实为材料,人工接种细交链孢菌,分别在接种0.5、1、2、3和4 d后取样,通过抑制性消减杂交技术(suppression subtractive hybridization,SSH)构建了病原菌诱导的差异表达的c DNA消减文库。【结果】PCR鉴定随机挑取的阳性克隆,显示插入片段大小为200~900 bp。随机挑选200个阳性克隆进行测序,利用BLASTx在Gen Bank Nr数据库进行序列比对分析,共获得118个Unigenes。对这些ESTs进行功能分类发现细交链孢菌侵染下诱导表达基因涉及植物细胞内的多种代谢和应答过程,其中包括抗病/防御类、信号传导途径类、新陈代谢类、蛋白质合成和加工类及细胞结构的组成等,尤其以抗病/防御类基因所占比例最大(27.17%)。【结论】通过SSH研究了缩果病病原侵染果实过程的差异表达基因,鉴定到一些与枣缩果病相关的基因,为进一步研究相关基因及今后筛选防治枣缩果病提供理论基础。
[Abstract]:[objective] to investigate the differentially expressed genes in the process of infection of jujube fruit by Streptomyces spp. [methods] the fruits of 'honeypot' jujube at white maturity were artificially inoculated with Streptomyces fine cross. The samples were collected 3 and 4 days after 0. 5, 1, 2, 3 and 4 days, respectively. The differentially expressed c DNA subtractive library induced by pathogenic bacteria was constructed by suppression subtractive hybrid (suppression subtractive hybridization,SSH). [results] the randomly selected positive clone was identified by PCR, and the size of the inserted fragment was 200 鈮，
本文编号：2485055
[Abstract]:[objective] to investigate the differentially expressed genes in the process of infection of jujube fruit by Streptomyces spp. [methods] the fruits of 'honeypot' jujube at white maturity were artificially inoculated with Streptomyces fine cross. The samples were collected 3 and 4 days after 0. 5, 1, 2, 3 and 4 days, respectively. The differentially expressed c DNA subtractive library induced by pathogenic bacteria was constructed by suppression subtractive hybrid (suppression subtractive hybridization,SSH). [results] the randomly selected positive clone was identified by PCR, and the size of the inserted fragment was 200 鈮，
本文编号：2485055
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