火鹤八氢番茄红素脱氢酶基因的克隆与其表达分析

发布时间：2019-06-01 08:35

【摘要】：旨在为火鹤叶色和花色呈色机理研究提供一定理论依据。以火鹤‘阿拉巴马’为材料,提取火鹤肉穗花序中的总RNA,采用RACE技术克隆得到了八氢番茄红素脱氢酶基因(Aa PDS),通过RT-PCR分析其表达变化。八氢番茄红素脱氢酶基因(Aa PDS)c DNA全长为2226 bp,其中开放阅读框(ORF)共1767个碱基,编码588个氨基酸。序列比对后发现火鹤Aa PDS序列与鹤望兰、水仙、烟草、葡萄、黑藻、向日葵等PDS氨基酸序列存在高度同源性,有74%~79%的一致性。系统进化分析表明火鹤Aa PDS与黑藻、水稻、水仙、鹤望兰等单子叶植物的PDS基因聚类在一起应用。RT-PCR分析表明,Aa PDS在肉穗花序中表达量最高,在佛焰苞中表达次之,在叶、茎、根表达量依次降低。以上结果可见,通过分子生物学进行系统分类与传统植物表观分类学结果一致。类胡萝素代谢途径中的PDS基因在火鹤花器官成色中起一定作用。
[Abstract]:The purpose of this paper is to provide a theoretical basis for the study of the color mechanism of fire crane leaf color and flower color. Using fire crane 'Alabama' as material, the total RNA, from the inflorescence of fire crane meat was extracted and cloned by RACE technique to obtain the expression change of octahydrolycopene dehydrogenase gene (Aa PDS),. RT-PCR analysis was used to analyze the expression of octahydrolycopene dehydrogenase gene from the inflorescence of fire crane 'Alabama'. The full length of octahydrolycopene dehydrogenase gene (Aa PDS) c DNA is 2226 bp,. The open reading frame (ORF) has 1767 bases and encodes 588 amino acids. After sequence alignment, it was found that the Aa PDS sequence of fire crane had high homology with PDS amino acid sequences such as Hewanglan, Narcissus, tobacco, grape, black algae, sunflower and so on, with a consistency of 74% and 79%. Systematic evolution analysis showed that fire crane Aa PDS was used in cluster with PDS gene of monocotyledonous plants such as black algae, rice, narcissus and Elaeagnus angustifolia. RT-PCR analysis showed that, Aa PDS was the highest in the inflorescence of meat panicle, followed by that in Buddha flame bud. The expression levels of leaves, stems and roots decreased in turn. The above results show that the results of systematic classification by molecular biology are consistent with those of traditional plant apparent taxonomy. PDS gene plays an important role in the color formation of flower organs of Crane.
【作者单位】：北京农学院园林学院;城乡生态环境北京实验室;北京市乡村景观规划设计工程技术研究中心;北京林果业生态环境功能提升协同创新中心;
【基金】：北京市科技提升计划(TJSHG201310020020) 北京市教委科技计划面上项目(KM201510020011) 科技创新服务能力建设-协同创新中心-林果业生态环境功能提升协同创新中心(2011协同创新中心)(市级)(PXM2016_014207_000038) 城乡生态环境北京实验室项目(PXM2016-014207-000003) 北京市属高等学校创新团队建设项目(IDHT20150503)
【分类号】：S682.14

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