HCN2基因和ANK2基因在心源性猝死病人窦房结细胞的表达变化
发布时间：2019-06-07 09:49
【摘要】：目的通过检测超极化激活环核苷酸门控阳离子通道2蛋白(HCN2)和细胞膜锚蛋白蛋白(ANK2)在心源性猝死(SCD)病人和非心源性猝死病人窦房结细胞的表达水平,初步探讨HCN2蛋白和ANK2蛋白在SCD病人窦房结的表达变化及其意义。方法收集青岛市市立医院病理科2015年9月-2016年3月诊断为SCD的个体心脏27例作为研究组,其中23例为冠心病患者,4例为病毒性心肌炎患者;选择因交通事故、外伤等所致死亡者标本13例作为对照组。准确切取心脏窦房结组织,石蜡包埋,部分HE染色,部分免疫组织化学染色,采用Mann-Whitney秩和检验、t检验和pearson相关系数进行统计分析。结果1.窦房结病理形态学观察:SCD组和非SCD组窦房结组织均存在不同程度的基础病理改变,包括脂肪浸润、纤维化、动脉狭窄、慢性炎症、大片出血等,SCD组窦房结病理改变数量及严重程度远高于非SCD组,差异有统计学意义(p=0.0110.01)。2.窦房结HCN2蛋白的表达:HCN2在窦房结组织P细胞及T细胞中均有表达,HCN2在SCD组及非SCD组中均呈核旁点状着色。SCD组HCN2在窦房结组织中呈棕褐色或棕黄色颗粒非均质分布在核旁;非SCD组HCN2不表达或微量表达,淡黄色细颗粒稀疏散在分布在核旁。SCD组窦房结组织HCN2蛋白表达量高于非SCD组,差异有统计学意义(p0.01)。3.窦房结ANK2蛋白的表达:ANK2在窦房结组织定位在细胞胞浆中,在P细胞及T细胞中均有表达。SCD组ANK2微量表达,稀疏分布于窦房结细胞胞浆内;非SCD组ANK2呈深黄色染色,均质分布于细胞浆内。SCD组ANK2蛋白表达量明显低于对照组,差异有统计学意义(p0.01)。4.窦房结HCN2蛋白及ANK2蛋白表达的相关性:用pearson等级相关分析心源性猝死组HCN2、ANK2蛋白表达的相关性显示,HCN2、ANK2蛋白的表达无明显相关性(r=-0.453,p=0.120.05)。结论心源性猝死患者窦房结均存在不同程度的病理改变,与正常对照组相比,HCN2蛋白表达水平上调,ANK2蛋白表达水平下调。我们的结果表明,离子通道蛋白表达紊乱可能导致严重心律失常从而成为发生心源性猝死的重要原因。意义本文章以人体心脏标本做研究,探讨了HCN2及ANK2通道蛋白在心源性猝死者窦房结组织中的表达水平,克服了以往的以动物模型为研究的局限性。实验结果表明,HCN2及ANK2在蛋白质水平的表达异常可能与SCD的发生相关,为不明原因性SCD的诊断提供了重要的参考价值,为病人寻找死因提供帮助,而且对早期预防及治疗心源性猝死有一定的帮助。
[Abstract]:Objective to detect the expression of hyperpolarization activated cyclic nucleotides gated cationic channel 2 protein (HCN2) and cell membrane anchor protein (ANK2) in sinoatrial node cells of patients with sudden cardiac death (SCD) and patients with non-cardiac sudden death. To investigate the expression and significance of HCN2 protein and ANK2 protein in sinoatrial node of SCD patients. Methods from September 2015 to March 2016, 27 individual hearts diagnosed as SCD were collected as the study group, including 23 patients with coronary heart disease and 4 patients with viral myocarditis. Thirteen cases of death caused by traffic accidents and trauma were selected as control group. Cardiac sinoatrial node tissue was accurately resected, paraffin embedded, partial HE staining and partial immunohistochemical staining. Mann-Whitney rank sum test, t test and pearson correlation coefficient were used for statistical analysis. Result 1. Pathomorphological observation of sinoatrial node: there were basic pathological changes in sinoatrial node tissue in SCD group and non-SCD group, including fat infiltration, fibrosis, arterial stenosis, chronic inflammation, massive hemorrhage and so on. The number and severity of pathological changes of sinoatrial node in SCD group were much higher than those in non-SCD group (p 鈮，
本文编号：2494703
[Abstract]:Objective to detect the expression of hyperpolarization activated cyclic nucleotides gated cationic channel 2 protein (HCN2) and cell membrane anchor protein (ANK2) in sinoatrial node cells of patients with sudden cardiac death (SCD) and patients with non-cardiac sudden death. To investigate the expression and significance of HCN2 protein and ANK2 protein in sinoatrial node of SCD patients. Methods from September 2015 to March 2016, 27 individual hearts diagnosed as SCD were collected as the study group, including 23 patients with coronary heart disease and 4 patients with viral myocarditis. Thirteen cases of death caused by traffic accidents and trauma were selected as control group. Cardiac sinoatrial node tissue was accurately resected, paraffin embedded, partial HE staining and partial immunohistochemical staining. Mann-Whitney rank sum test, t test and pearson correlation coefficient were used for statistical analysis. Result 1. Pathomorphological observation of sinoatrial node: there were basic pathological changes in sinoatrial node tissue in SCD group and non-SCD group, including fat infiltration, fibrosis, arterial stenosis, chronic inflammation, massive hemorrhage and so on. The number and severity of pathological changes of sinoatrial node in SCD group were much higher than those in non-SCD group (p 鈮，
本文编号：2494703
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