ABA对盐胁迫下侧柏活性氧代谢及其相关基因表达的研究

发布时间：2019-06-17 21:13

【摘要】：以侧柏幼苗为试验材料,采用水培方式,研究不同浓度NaCl(0、100、200、300和400mmol·L~(-1))以及ABA(0、0.5、1、10、100和200μmol·L~(-1)处理对盐胁迫下侧柏幼苗活性氧代谢的影响。结果显示:(1)随着NaCl处理浓度增加,侧柏幼苗叶片过氧化氢(H_2O_2)、丙二醛(MDA)、谷胱甘肽(GSH)和脯氨酸含量,以及抗氧化物酶(SOD、POD和CAT)活性呈上升趋势,而可溶性蛋白含量降低。(2)于300mmol·L~(-1)NaCl胁迫处理48h后进行ABA(1和10μmol·L~(-1))处理,分析发现侧柏幼苗叶片的SOD、POD和CAT活性提高,GSH、脯氨酸和可溶性蛋白含量增加,H_2O_2和MDA的积累减少。(3)NaCl胁迫后,侧柏幼苗叶片活性氧代谢相关基因(Cu/Zn-SOD、CAT、GR、APX、MDAR和GST)的表达水平随ABA浓度增加呈先升后降的趋势,并于10μmol·L~(-1) ABA处理48h时达到峰值。研究表明,适宜浓度ABA通过促进盐胁迫下侧柏幼苗叶片的抗氧化物酶活性,提高渗透调节能力,增加GSH含量,降低H_2O_2和MDA积累,从而有效降低活性氧对侧柏叶片的伤害,保护细胞膜结构的完整,增强其抗盐性。
[Abstract]:Using Platycladus orientalis seedlings as experimental materials, the effects of different concentrations of NaCl (0100200300 and 400mmol 路L ~ (- 1) and ABA (0, 0.5, 1, 10100 and 200 渭 mol 路L ~ (- 1) on reactive oxygen species metabolism in Platycladus orientalis seedlings under salt stress were studied. The results showed that: (1) with the increase of NaCl concentration, the contents of hydrogen peroxide (H_2O_2), malondialdehyde (MDA), glutathione (GSH) and proline, and the activities of antioxidant enzymes (SOD,POD and CAT) in leaves of Platycladus orientalis seedlings increased, while the soluble protein content decreased. (2) treated with 300mmol 路L ~ (- 1) NaCl stress for 48 h, ABA (1 and 10 渭 mol 路L ~ (- 1) was treated with ABA (1 and 10 渭 mol 路L ~ (- 1). The results showed that the activities of SOD,POD and CAT increased, the contents of GSH, proline and soluble protein increased, and the accumulation of H_2O_2 and MDA decreased after NaCl stress. (3) after NaCl stress, the expression of active oxygen metabolism related genes (Cu/Zn-SOD,CAT,GR,APX,MDAR and GST) in leaves of Platycladus orientalis seedlings increased at first and then decreased with the increase of ABA concentration, and reached the peak at 48 h after treatment with 10 渭 mol 路L ~ (- 1) ABA. The results showed that the suitable concentration of ABA could effectively reduce the damage of reactive oxygen species to Platycladus orientalis leaves, protect the integrity of cell membrane structure and enhance its salt resistance by promoting antioxidant enzyme activity, increasing osmotic regulation ability, increasing GSH content and decreasing the accumulation of H_2O_2 and MDA in leaves of Platycladus orientalis seedlings under salt stress.
【作者单位】：中国林业科学研究院林业研究所国家林业局林木培育重点实验室;阜阳师范学院生物与食品工程学院;
【基金】：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2014QB004) 国家自然科学基金(31300555) 安徽省哲学社会科学规划项目(AHSKQ2016D110)
【分类号】：Q945.78;S791.38

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