合浦珠母贝海洋酸化和温度胁迫下基因共表达网络分析

发布时间：2019-06-19 15:29

【摘要】：利用共表达网络分析法(WGCNA)筛选出合浦珠母贝外套膜在高温胁迫下影响显著的1个响应基因模块和海水酸化胁迫下的5个响应基因模块,并对模块中包含的基因进行功能富集;分析贝壳蛋白与模块其他基因的关联性,构建矿化基因的应激网络。高温显著响应模块基因富集结果显示细胞骨架蛋白发生了显著的变化,如ARHGEF4、SAP和myosin。酸化应答模块共5个,响应的基因包括呼吸相关的血红素A合成酶、芳香族氨基酸代谢相关的酶、钙粘蛋白和血小板反应蛋白等。另外,高温应答模块聚集了最多矿化基因,包括MSI、Tyrosinase、Fibronectin-like protein等重要的矿化基因,说明这些矿化基因对温度较为敏感。构建基因共表达网络的结果显示钙离子结合蛋白、细胞骨架蛋白、蛋白质糖基化相关的基因关系密切。
[Abstract]:using the co-expression network analysis method (WGCNA) to screen out the five response gene modules under high temperature stress and the five response gene modules under the seawater acidification stress, and carrying out function enrichment on the genes contained in the module; The relationship between the shell protein and other genes of the module is analyzed, and the stress network of the mineralization gene is constructed. The high temperature significant response module gene enrichment results in significant changes in the cell skeletal protein, such as ARHGEF4, SAP, and myosin. The response genes include respiration-related heme A synthetase, the enzyme related to the metabolism of aromatic amino acid, the calcium-binding protein and the platelet-reactive protein. In addition, the high-temperature response module gathers the most mineralization genes, including the important mineralization genes such as MSI, Tyrosinase, and Fironectin-like protein, indicating that these mineralization genes are sensitive to the temperature. The results of the gene co-expression network show that the calcium ion binding protein, the cytoskeleton protein and the protein glycosylation related genes are closely related.
【作者单位】：中国科学院大学广东海洋大学水产学院广东省珍珠养殖与加工工程技术研究中心中国科学院海洋研究所
【基金】：现代农业产业技术体系建设专项资金(CARS-48)项目山东省泰山学者攀登计划共同资助
【分类号】：S917.4

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