玉米ZmPGP1基因启动子的克隆及结构功能分析
发布时间：2019-06-20 13:54
【摘要】：为分析玉米ZmPGP1基因启动子的功能,利用巢式PCR方法克隆出了玉米ZmPGP1基因的启动子调控区,并将该启动子与GUS基因融合,通过基因枪法转入玉米(Zea mays)中,分析ZmPGP1启动子表达特性。结果显示,在玉米中克隆出ZmPGP1基因5′端上游1 090bp的启动子序列,该启动子序列包括光响应元件、激素响应元件和胁迫诱导及发育相关顺式作用元件。GUS染色表明ZmPGP1基因在玉米幼苗的茎部、叶子及根中都有表达,其中茎的节间处以及叶鞘部位表达量较高,这与ZmPGP1基因的Real-time PCR分析结果一致。研究结果进一步阐明ZmPGP1基因的功能以及作用机理。
[Abstract]:In order to analyze the function of the maize ZmPGP1 gene promoter, the promoter regulatory region of the maize ZmPGP1 gene was cloned by a nested PCR method, and the promoter was fused with the GUS gene. The expression of the ZmPGP1 promoter was analyzed by the gene gun method. The results showed that the promoter sequence of 1 090 bp upstream of the 5-terminal end of the ZmPGP1 gene was cloned in the maize, and the promoter sequence included light response element, hormone response element and stress-inducing and developing-related cis-acting element. The GUS staining showed that the ZmPGP1 gene was expressed in the stem, leaf and root of the maize seedling, and the expression of the internode of the stem and the leaf-leaf part was high, which was consistent with the results of the Real-time PCR analysis of the ZmPGP1 gene. The results of the study further illustrate the function and mechanism of the ZmPGP1 gene.
【作者单位】： 江苏省农垦农业科学研究院;中国农业大学农学院;
【基金】：国家自然科学基金项目(31171564) 公益性行业(农业)科技专项(201303002)
【分类号】：S513
，
本文编号：2503273
[Abstract]:In order to analyze the function of the maize ZmPGP1 gene promoter, the promoter regulatory region of the maize ZmPGP1 gene was cloned by a nested PCR method, and the promoter was fused with the GUS gene. The expression of the ZmPGP1 promoter was analyzed by the gene gun method. The results showed that the promoter sequence of 1 090 bp upstream of the 5-terminal end of the ZmPGP1 gene was cloned in the maize, and the promoter sequence included light response element, hormone response element and stress-inducing and developing-related cis-acting element. The GUS staining showed that the ZmPGP1 gene was expressed in the stem, leaf and root of the maize seedling, and the expression of the internode of the stem and the leaf-leaf part was high, which was consistent with the results of the Real-time PCR analysis of the ZmPGP1 gene. The results of the study further illustrate the function and mechanism of the ZmPGP1 gene.
【作者单位】： 江苏省农垦农业科学研究院;中国农业大学农学院;
【基金】：国家自然科学基金项目(31171564) 公益性行业(农业)科技专项(201303002)
【分类号】：S513
，
本文编号：2503273
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