番茄HD-Zip转录因子家族生物信息学分析及HD-ZipⅠ亚族抗逆相关基因鉴定分析

发布时间：2019-06-22 10:53

【摘要】：随着番茄种植规模的不断扩大,高盐、干旱、低温、病原菌等各种非生物和生物胁迫对番茄的生长及生产造成了严重的危害。目前为止,大量研究显示,植物转录因子在植物适应和抵御各种非生物及生物胁迫的过程中起着重要的调控作用。近年来对于植物不同转录因子家族的研究越来越深入,HD-Zip是植物中特有的一类转录因子,参与植物多种生物功能,尤其对植物在非生物胁迫下的应答反应有着重要调控作用。在本研究中,对番茄HD-Zip转录因子基因家族进行了生物信息学分析,对番茄HDZip蛋白家族的成员进行了分类、理化特性、保守区、基因结构、染色体分布、二级和三级结构的分析。同时对番茄HD-Zip I亚家族组织特异性及非生物胁迫下的表达模式进行了分析,对应答表现明显的两个基因进行了基因沉默分析。为番茄HD-Zip基因家族在非生物胁迫应答过程中的调控功能研究提供理论依据。主要研究结果如下:鉴定出番茄HD-Zip转录因子家族共包含58个成员(Sl HZ1-Sl HZ58),分为SlHD-ZipⅠ-Ⅳ亚家族。这些基因不均匀的分布在12条染色体上,番茄HD-Zip基因扩增方式主要为片段复制,家族成员的二级结构主要为螺旋,三级结构具有相似性。对HD-Zip I亚族15个基因进行了组织特异性表达分析,结果显示,这些成员在根、茎、叶、花以及果实中均有不同程度的表达。大部分基因都能在根中进行表达,而Sl HZ30基因仅在花中表达。逆境胁迫表达分析中,在盐胁迫处理下14个基因表达上调,1个基因(SlHZ14)表达下调。在干旱处理中13个基因表达上调,2个基因(Sl HZ14和SlHZ28)表达下调。在低温处理中全部15个基因表达上调,其中SlHZ13基因上调最为显著。对逆境胁迫应答分析中表达量变化显著的基因Sl HZ11和SlHZ15进行了VIGS基因沉默分析,发现SlHZ11和Sl HZ15基因沉默后,植株在盐和干旱逆境条件下的MDA指标与相同条件下的正常植株相比显著上升,说明该基因的下调表达可以降低植株的耐盐和抗旱性。
[Abstract]:With the continuous expansion of tomato planting scale, high salt, drought, low temperature, pathogenic bacteria and other abiotic and biological stresses have caused serious harm to the growth and production of tomato. So far, a large number of studies have shown that plant transcription factors play an important role in plant adaptation and resistance to various abiotic and biological stresses. In recent years, the study of different family of transcription factors in plants has been more and more in-depth. HD-Zip is a unique transcription factor in plants, which is involved in a variety of biological functions of plants, especially plays an important role in regulating the response of plants under abiotic stress. In this study, the HD-Zip transcription factor gene family of tomato was analyzed by bioinformatics, and the members of tomato HDZip protein family were classified, physicochemical characteristics, conserved region, gene structure, chromosome distribution, secondary and tertiary structure. At the same time, the tissue specificity of tomato HD-Zip I subfamily and the expression pattern under abiotic stress were analyzed, and the gene silencing analysis of two genes with obvious response was carried out. It provides a theoretical basis for the study of the regulatory function of tomato HD-Zip gene family in abiotic stress response. The main results are as follows: a total of 58 members (Sl HZ1-Sl HZ58) of tomato HD-Zip transcription factor family were identified, which were divided into SlHD-Zip I-IV subfamily. These genes were unevenly distributed on 12 chromosomes. The amplification mode of tomato HD-Zip gene was mainly fragment replication, the secondary structure of family members was mainly spiral, and the tertiary structure was similar. The tissue specific expression of 15 genes of HD-Zip I subfamily was analyzed. the results showed that these members were expressed in roots, stems, leaves, flowers and fruits to varying degrees. Most genes can be expressed in roots, while Sl HZ30 genes can only be expressed in flowers. In stress analysis, 14 genes were up-regulated and 1 gene (SlHZ14) was down-regulated under salt stress. In drought treatment, 13 genes were up-regulated and 2 genes (Sl HZ14 and SlHZ28) were down-regulated. The expression of all 15 genes was up-regulated in low temperature treatment, and the up-regulation of SlHZ13 gene was the most significant. VIGS gene silencing analysis of genes SlHZ11 and SlHZ15 with significant changes in stress response analysis was carried out. It was found that after SlHZ11 and SlHZ15 gene silencing, the MDA index of plants under salt and drought stress was significantly higher than that of normal plants under the same conditions, which indicated that the down-regulated expression of SlHZ11 gene could decrease the salt tolerance and drought resistance of plants.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S641.2

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