靶向Hiwi基因的siRNA干扰对诱导三阴乳腺癌MDA-MB-231细胞凋亡的影响

发布时间：2019-06-26 08:44

【摘要】：目的:探讨干扰Hiwi基因对三阴乳腺癌细胞MDA-MB-231凋亡的影响。方法:培养并转染三阴乳腺癌细胞MDA-MB-231,按照实验设计分为6组,转染后应用q RT-PCR、Western blot分析Hiwi基因的m RNA及其靶蛋白的表达情况,根据结果评估干扰效果筛选有效的si RNA干扰片段作为干扰组(Hiwi10330组),再按照试验设计分为3组分别是干扰组,阴性对照组(NC组)、空白对照组(Blank组),在细胞转染后进行细胞流式检测各实验组细胞凋亡率。结果:靶向Hiwi基因的si RNA干扰后,干扰组中m RNA的表达量明显降低(P0.05),Hiwi基因靶蛋白的表达也出现了明显的抑制(P0.05)。靶向Hiwi基因的si RNA干扰后,干扰组MDA-MB-231细胞的凋亡率与NC和Blank组相比明显升高(P0.05)。结论:si RNA靶向沉默Hiwi基因后,乳腺癌细胞MDA-MB-231的凋亡率明显升高,提示Hiwi基因可能成为三阴乳腺癌的一潜在治疗靶点。
[Abstract]:Objective: to investigate the effect of interfering Hiwi gene on apoptosis of Sanyin breast cancer cell line MDA-MB-231. Methods: Sanyin breast cancer cell line MDA-MB-231, was cultured and transformed into 6 groups according to the experimental design. The expression of m RNA and its target protein of Hiwi gene was analyzed by Q RT-PCR,Western blot. According to the results, the effective si RNA interference fragments were selected as interference group (Hiwi10330 group), and then divided into three groups according to the experimental design: interference group, negative control group (NC group) and blank control group (Blank group). The apoptosis rate of each experimental group was detected by flow cytometry after cell transfer. Results: after si RNA interference of targeted Hiwi gene, the expression of m RNA in interference group was significantly decreased (P 0.05), and the expression of target protein of), Hiwi gene was also significantly inhibited (P 0.05). After si RNA interference targeting Hiwi gene, the apoptosis rate of MDA-MB-231 cells in interference group was significantly higher than that in NC and Blank groups (P 0.05). Conclusion the apoptosis rate of breast cancer cell line MDA-MB-231 increased significantly after: si RNA targeted silencing of Hiwi gene, suggesting that Hiwi gene may be a potential therapeutic target for Sanyin breast cancer.
【作者单位】：广东医学院附属医院肿瘤中心四区;广东医学院附属医院乳腺外科;湛江市第四人民医院乳腺科;
【基金】：广东省湛江市科技攻关计划项目(编号:2015B01029)
【分类号】：R737.9

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