转2mG2-epsps基因水稻种子蛋白质非预期变异分析

发布时间：2019-07-09 18:06

【摘要】：以转2mG2-epsps基因抗草甘膦水稻为材料,根据实质等同性原则,分析转基因(genetically modified,GM)水稻种子可溶性蛋白含量和组分的非预期变异。结果表明:与非转基因亲本相比,GM水稻种子的总可溶性蛋白含量极显著降低,主要受谷蛋白含量变异影响。但清、球、醇、谷4种蛋白含量的最高变异幅度(9.12%)低于P1和P2两亲本之间的最大差异幅度(14.67%)。说明GM水稻种子的可溶性蛋白含量非预期变异在安全范围内,但营养品质可能与亲本品系存在差异。SDS-PAGE分析发现,GM水稻株系T12和T22分别有12和14个蛋白谱带发生了非预期变异,涉及4种可溶性蛋白的组分,多数表现为含量变化。突出的是,2个GM水稻株系中均新增加了41 ku和56 ku谷蛋白组分,需要进一步通过毒理学分析评价其食品安全性。
[Abstract]:2mG2-epsps transgenic glyphosate resistant rice was used as material to analyze the unexpected variation of soluble protein content and components in transgenic (genetically modified,GM) rice seeds according to the principle of substantive equivalence. The results showed that the total soluble protein content of GM rice seeds was significantly lower than that of non-transgenic parents, which was mainly affected by the variation of gluten content. However, the highest variation range of protein content among Qing, ball, alcohol and valley (9.12%) was lower than that between P1 and P2 parents (14.67%). The results showed that the unexpected variation of soluble protein content in GM rice seeds was within a safe range, but the nutritional quality might be different from that of parent lines. SDS-PAGE analysis showed that 12 and 14 protein bands of GM rice lines T12 and T22 had unexpected variation, involving four soluble protein components, most of which showed content changes. It is prominent that 41 ku and 56 ku glutelin components have been added to the two GM rice lines, which needs to be further evaluated by toxicological analysis.
【作者单位】：浙江工商大学食品与生物工程学院;
【基金】：国家自然科学基金(30871511) 农业部转基因生物新品种培育重大专项(2011ZX 08010-003)
【分类号】：S511

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