高粱Pht1基因家族的鉴定及其进化和表达分析
发布时间：2019-07-13 22:16
【摘要】：磷是生物大分子的重要组成成分,也是植物生长所必需的重要营养元素。利用Phytozome数据库,通过拟南芥Pht1家族、水稻OsPht1家族基因序列在高粱全基因组中的同源比对,鉴定并获得了11个高粱Pht1家族成员的基因序列、基因结构、蛋白序列和染色体定位等信息。利用荧光定量的方法,检测了11个SbPht1基因在缺磷和高磷处理下的表达差异。结果表明,在缺磷条件下,大部分SbPht1基因表达量上升。高磷胁迫下,SbPT1、SbPT4、SbPT6、SbPT7、SbPT8、SbPT9和SbPT10在根中的表达量上升,SbPT3和SbPT5在地上部的表达量上升。分析高粱全基因组磷转运蛋白,对于研究植物在磷吸收过程中的分子生物学基础,改良植物吸收和利用磷素意义重大。
[Abstract]:Phosphorus is not only an important component of biological macromolecules, but also an important nutrient element for plant growth. Using Phytozome database, the gene sequences, gene structure, protein sequence and chromosome location of 11 sorghum Pht1 family members were identified and obtained by homologous alignment of Arabidopsis Pht1 family and rice OsPht1 family gene sequence in sorghum genome. The expression difference of 11 SbPht1 genes under phosphorus deficiency and high phosphorus treatment was detected by fluorescence quantitative method. The results showed that the expression of most SbPht1 genes increased under the condition of phosphorus deficiency. Under high phosphorus stress, the expression of SbPT1,SbPT4,SbPT6,SbPT7,SbPT8,SbPT9 and SbPT10 in roots increased, while the expression of SbPT3 and SbPT5 increased in shoot. It is of great significance to analyze the whole genome phosphorus transporter of sorghum in order to study the molecular biological basis of phosphorus absorption in plants and to improve the absorption and utilization of phosphorus in plants.
【作者单位】： 浙江省农业科学院蔬菜研究所;浙江大学生命科学学院;
【基金】：国家基金面上项目(51375460) 浙江省农业科学院青年人才培养项目(2016R23R08E05);浙江省农业科学院创新载体建设工程项目
【分类号】：S514
本文编号：2514329
[Abstract]:Phosphorus is not only an important component of biological macromolecules, but also an important nutrient element for plant growth. Using Phytozome database, the gene sequences, gene structure, protein sequence and chromosome location of 11 sorghum Pht1 family members were identified and obtained by homologous alignment of Arabidopsis Pht1 family and rice OsPht1 family gene sequence in sorghum genome. The expression difference of 11 SbPht1 genes under phosphorus deficiency and high phosphorus treatment was detected by fluorescence quantitative method. The results showed that the expression of most SbPht1 genes increased under the condition of phosphorus deficiency. Under high phosphorus stress, the expression of SbPT1,SbPT4,SbPT6,SbPT7,SbPT8,SbPT9 and SbPT10 in roots increased, while the expression of SbPT3 and SbPT5 increased in shoot. It is of great significance to analyze the whole genome phosphorus transporter of sorghum in order to study the molecular biological basis of phosphorus absorption in plants and to improve the absorption and utilization of phosphorus in plants.
【作者单位】： 浙江省农业科学院蔬菜研究所;浙江大学生命科学学院;
【基金】：国家基金面上项目(51375460) 浙江省农业科学院青年人才培养项目(2016R23R08E05);浙江省农业科学院创新载体建设工程项目
【分类号】：S514
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