国内外转基因检测能力验证概述
发布时间：2019-07-22 20:47
【摘要】：转基因检测以外源DNA、蛋白质等为检测对象,以定性和定量PCR技术、酶联免疫吸附技术为主要检测手段,最终判断样品中转基因成分组成及其含量。进行转基因检测的机构是转基因生物安全监管的重要技术支撑,应当确保检测结果可靠、准确。能力验证是实验室内部和外部质量控制的有效手段,监管机构借此来确定检测实验室的能力和水平。当前,中国转基因检测能力验证的组织水平与国外相比还有较大差距。介绍了国外一些机构如Fapas、GIPSA、ISTA组织转基因能力验证工作的流程,其共同点都是盲样检测,对阳性成分进行定值,并以离散度来判定结果。同时,介绍了中国农业部和质检系统组织能力验证的通常做法,以期提出进一步加强我国转基因检测能力验证工作的对策和建议。
[Abstract]:Transgenic detection took exogenous DNA, protein as the detection object, qualitative and quantitative PCR technology, enzyme-linked immunosorbent assay (Elisa) as the main detection methods, and finally judged the composition and content of transgenic components in the sample. The organization carrying out transgenic detection is an important technical support for the safety supervision of genetically modified organisms, and the detection results should be reliable and accurate. Capability verification is an effective means of internal and external quality control in the laboratory, which is used by regulators to determine the ability and level of the testing laboratory. At present, there is still a big gap between China and foreign countries in the organization level of transgenic detection ability verification. This paper introduces the process of verifying transgenic ability of some foreign organizations, such as Fapas,GIPSA,ISTA. The common points are blind sample detection, the positive components are valued, and the dispersion is used to determine the results. At the same time, this paper introduces the common methods of organization ability verification of Chinese Ministry of Agriculture and quality inspection system, in order to put forward some countermeasures and suggestions to further strengthen the verification of transgenic detection ability in China.
【作者单位】： 农业部科技发展中心;
【基金】：“十三五”国家科技重大专项(2016ZX08012003)资助
【分类号】：Q78
本文编号：2517888
[Abstract]:Transgenic detection took exogenous DNA, protein as the detection object, qualitative and quantitative PCR technology, enzyme-linked immunosorbent assay (Elisa) as the main detection methods, and finally judged the composition and content of transgenic components in the sample. The organization carrying out transgenic detection is an important technical support for the safety supervision of genetically modified organisms, and the detection results should be reliable and accurate. Capability verification is an effective means of internal and external quality control in the laboratory, which is used by regulators to determine the ability and level of the testing laboratory. At present, there is still a big gap between China and foreign countries in the organization level of transgenic detection ability verification. This paper introduces the process of verifying transgenic ability of some foreign organizations, such as Fapas,GIPSA,ISTA. The common points are blind sample detection, the positive components are valued, and the dispersion is used to determine the results. At the same time, this paper introduces the common methods of organization ability verification of Chinese Ministry of Agriculture and quality inspection system, in order to put forward some countermeasures and suggestions to further strengthen the verification of transgenic detection ability in China.
【作者单位】： 农业部科技发展中心;
【基金】：“十三五”国家科技重大专项(2016ZX08012003)资助
【分类号】：Q78
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2 杨瑾瑞;微生物能力验证实验结果分析[J];职业卫生与病伤;2005年03期
3 游勇来;赵琼;陈志耿;;能力验证样品中沙门氏菌的分离与鉴定[J];检验检疫学刊;2013年01期
，本文编号：2517888
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