不同生育期组大豆品种GmCOL家族基因的自然变异

发布时间：2020-12-18 04:24

　　适时开花和成熟对于大豆来说是重要的生态和农艺性状,这决定了在不同环境下大豆的适应性、生长发育、品质及产量等。自然变异是进化的主要来源,它决定了一个物种的适应潜力。多数自然变异呈现数量性状,由多个位点和基因的分子遗传多样性决定。在光周期调控的开花和发育过程中,BBX基因家族成员CONSTANS（CO）是中心调控基因,引发可移动成花素FT基因的表达,进而使得作物进入开花阶段。在大豆中,CONSTANS-LIKE基因不仅在开花阶段起作用,在其他重要生理过程中也发挥了重要作用。然而,COL家族基因的自然变异与大豆开花和成熟的关系尚不明确。本研究中,选取来自14个生育期组（MG0000-MGX）的128个大豆品种作为试验材料。材料种植于中国北京的自然光照条件下,并对各生育时期-出苗期（VE）、初花期（R1）和完熟期（R8）进行调查。供试大豆品种的开花期和成熟期具有显著差异。根据基因型和生长发育结构,供试品种分为3组MG0000-MG0,MGI-MGV和MGVI-MGX。在开花期（VE-R1）方面,生育期组MG0000,MGIX+X和MGVII的大豆品种开花期差异十分显著;成熟期（VE-R7）方面... 

【文章来源】：中国农业科学院北京市

【文章页数】：175 页

【学位级别】：博士

【文章目录】：
附件
摘要
Abstract
LIST OF ABBREVIATIONS
1 Chapter 1 Introduction
    1.1 Preamble
    1.2 Natural variation, flowering time, and maturity trait in soybean
    1.3 Photoperiodism phenomenon- remarkable findings in plant science
    1.4 Regulatory networks and genes influencing flowering time in plants
    1.5 Establishment of soybean growth season
    1.6 The purpose and significance of this study
2 Chapter 2 GmCOL Genes in Maturity and Flowering Time
    2.1 Photoperiod and relevant genes that influencing maturity and flowering
    2.2 CONSTANS and CONSTANS-Like genes
    2.3 Functional and evolutionary role of COL family gene in soybean
    2.4 Flowering time regulated by day length
    2.5 Co-relation between maturity, flowering and CONSTANS genes
    2.6 Regulatory role of COL family genes in photoperiod responsive in flowering in different species
    2.7 Identification and selection of determinate growth habit MG cultivars
3 Chapter 3 Materials and Methods
    3.1 Phenotype investigation of soybean in different maturity groups
        3.1.1 Experimental materials
        3.1.2 Methods
        3.1.3 Phenotypic evaluation
        3.1.4 Growth traits
        3.1.5 Phenotypic evaluation of stem growth habits
    3.2 Genetic Study
        3.2.1 Plant materials
        3.2.2 Genetic materials
        3.2.3 Gene identity of 21 GmCOL family genes
        3.2.4 Genomic DNA extraction
        3.2.5 Data mining and primer design
        3.2.6 PCR amplification and purification
        3.2.7 Sequencing and sequence data analysis
4 Chapter 4 Results and Analysis
    4.1 Analysis of soybean varieties in different growth stages
        4.1.1 Investigation results of growth period traits
        4.1.2 Association of flowering and maturity dates of soybean shown divergence
        4.1.3 Clustering analysis
        4.1.4 Analysis of soybean maturity group varieties growth period
        4.1.5 Soybean varieties matured differently with different beginning bloom days
    4.2 Bioinformatics analysis
        4.2.1 Construction and analysis of phylogram of COL genes in soybean
        4.2.2 Analysis and association of soybean determinacy
    4.3 Sequencing results: Analysis of COL genes in soybean varieties with different growth stages
        4.3.1 Gene sequencing results and genotype analysis
        4.3.2 Genotype distribution of GmCOL family genes in soybean cultivars
        4.3.3 Genotype number and genotype frequency of GmCOL genes
        4.3.4 GmCOL genes maturity groups and genotype distribution
        4.3.5 Statistical analysis of genotypes and phenotypes
        4.3.6 Association analysis GmCOL genotypes and pairwise comparison effect
        4.3.7 Soybean COL gene family exhibited different linkage disequilibrium
        4.3.8 Haplotype analysis of 21 soybean GmCOL family genes
        4.3.9 Haplotypes and natural variation of flowering time of GmCOL family genes…
5 Chapter 5 Discussion
    5.1 Selected soybean cultivars from different maturity groups show diversity in flowering time and maturity
    5.2 Natural variation, growth period group system and genotypes at the GmCOL loci of accessions
    5.3 Conservation and divergence of TFL1 homologs in soybean and other species
    5.4 Polymorphism of GmCOL family genes – association to flowering time and maturity…
6 Chapter 6 Summery and Conclusion
7 Chapter 7 References
Appendixes
Resume
Acknowledgements



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