水稻耐盐性QTL分析和盐胁迫下芽长候选基因qSL7的精细定位
发布时间：2022-02-11 13:26
盐分是限制全球水稻产量的主要非生物胁迫因素之一。长期的盐胁迫会引起水稻植株慢性离子毒害和水分渗透失衡,并导致死亡。水稻是全球三大主要粮食作物之一,因此,研究水稻在不同胁迫水平下耐盐性的遗传机制将有助于水稻抗逆育种。本研究,我们调查了超级杂交稻两优培九（LYP9）的亲本93-11和Pei-ai64s（PA64s）衍生的132个重组自交系（RIL）的耐盐性。进行了盐胁迫下数量性状基因座（QTL）和全基因组转录组分析,以及新主效QTL的精细定位。该研究的结果如下:一.鉴定了38个与盐胁迫相关的QTL。为了确定水稻盐分胁迫耐受性的遗传基础,在两种盐浓度处理下,对6个相关性状的QTL进行了定位。基于132个RIL的高密度遗传连锁图谱,我们对50和100 mM NaCl胁迫下的6个重要农艺性状进行了QTL分析,包括茎长（SL）、根长（RL）、茎鲜重（SFW）、根鲜重（RFW）、茎干重量（SDW）和根干重（RDW）。共定位到38个与6个性状相关的QTL,分布在1、2、3、4、5、6、7和10号染色体上。在2种胁迫水平下,共有19个QTL分布在5个QTL簇中。特别是,在两种盐浓度水平下,都检测到一个控...
【文章来源】：中国农业科学院北京市
【文章页数】：109 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
LIST OF ABBREVIATIONS
CHAPTER 1 INTRODUCTION
1.1 General Introduction
1.2 Review of literature
1.2.1 Rice:present status
1.2.2 Salinity stress effects on rice production
1.2.3 Salinity stress effects on rice plants
1.3 Salinity stress sensing in rice plant
1.4 Response of rice under salinity
1.4.1 Morpho-physiological response
1.4.2 Response at the biochemical level
1.4.3 Response at molecular level
1.5 Defense system of rice against salinity stress
1.6 Transcriptional regulation and gene expression of salinity tolerance
1.7 Molecular genetic mapping analysis of QTLs
1.8 Genetics and QTL mapping of salinity tolerance
CHAPTER 2 Mapping Quantitative Trait Loci Associated with Salinity Tolerance in RIL Population of Rice(Oryza sativa L.)
2.1 Introduction
2.2 Materials and methods
2.2.1 Plant materials and population development
2.2.2 Treatment of salt stress
2.2.3 Phenotypic evaluation of parents and progenies
2.2.4 Statistical analysis
2.2.5 Construction of linkage map and QTL analysis
2.3 Results
2.3.1 Evaluation of phenotypic characteristics under salt stress
2.3.2 Correlation analysis of traits
2.3.3 Identification of QTLs for seedling-stage salt tolerance traits
2.4 Discussion
2.5 Conclusion
CHAPTER 3 Comparative Transcriptome Profiling of93-11 and PA64s and Identification of Candidate Genes Involved in Salinity Tolerance
3.1 Introduction
3.2 Materials and methods
3.2.1 Plant growth conditions and salinity stress treatment
3.2.2 Phenotypic evaluation of seedlings under salt stress treatment
3.2.3 RNA extraction and c DNA library preparation and sequencing
3.2.4 Data filtering and assembly
3.2.5 Analysis and functional annotation of DEGs
3.2.6 Preparation of RNA
3.2.7 Synthesis of c DNA
3.3 Results
3.3.1 PA64s seedlings are more tolerant to salinity than93-11 seedlings
3.3.2 m RNA sequencing and functional annotation
3.3.3 Expression profiling analysis in the PA64s and93-11 under salinity
3.3.4 Gene ontology(GO)enrichment classification of the DEGs
3.3.5 Identification of differential transcription factor(TFs)
3.3.6 KEGG pathway enrichment analysis of the DEGs
3.3.7 Identification of candidate genes within the stable QTLs via RNA-seq
3.4 Discussion
3.5 Conclusion
CHAPTER 4 Genetic Dissection of Major Quantitative Trait Locus q SL7 for Shoot Length under Salinity Stress in Rice(Oryza sativa L.)
4.1 Introduction
4.2 Materials and methods
4.2.1 Plant materials and population development
4.2.2 Growing conditions
4.2.3 Phenotypic evaluation of93-11,CSSL-q SL7 and mapping populations
4.2.4 Na+/K+ion measurement
4.2.5 Photosynthetic pigment content measurement
4.2.6 Determination of soluble sugar
4.2.7 Determination of proline
4.2.8 DNA isolation and PCR analysis
4.2.9 Statistical analysis of data
4.3 Result
4.3.1 CSSL-q SL7 shows more salt tolerant than93-11
4.3.2 Photosynthetic capacity in93-11 and CSSL-qSL7
4.3.3 Accumulation of soluble sugar and proline in93-11 and CSSL-q SL7
4.3.4 Fine mapping of qSL7
4.4 Discussion
4.5 Conclusion
CHAPTER 5 Major findings and future perspectives
5.1 Major findings
5.2 Future perspectives
REFERENCES
APPENDIX
LIST OF PUBLICATIONS
ACKNOWLEDGEMENT
AUTHOR'S RESUME
【参考文献】：
期刊论文
[1]水稻耐盐基因定位与克隆及品种耐盐性分子标记辅助选择改良研究进展[J]. 井文,章文华. 中国水稻科学. 2017(02)
[2]Selection of Rice Genotypes for Salinity Tolerance Through Morpho-Biochemical Assessment[J]. Md. Nasim ALI,Bhaswati GHOSH,Saikat GANTAIT,Somsubhra CHAKRABORTY. Rice Science. 2014(05)
[3]Dissection of genetic overlap of salt tolerance QTLs at the seedling and tillering stages using backcross introgression lines in rice[J]. ZANG JinPing 1 ,SUN Yong 1 ,WANG Yun 1 ,YANG Jing 1 ,LI Fang 1 ,ZHOU YongLi 1 ,ZHU LingHua 1 , Reys JESSICA2,Fotokian MOHAMMADHOSEIN 2,XU JianLong 1&LI ZhiKang 1,2 1Institute of Crop Sciences/National Key Facility for Crop Gene Resources&Genetic Improvement,Chinese Academy of Agricul- tural Sciences,Beijing 100081,China; 2International Rice Research Institute,DAPO Box 7777,Metro Manila,Philippines. Science in China（Series C:Life Sciences）. 2008(07)
[4]高质量植物基因组DNA的分离[J]. 罗志勇,周钢,陈湘晖,陆秋恒,胡维新. 湖南医科大学学报. 2001(02)
本文编号：3620363
【文章来源】：中国农业科学院北京市
【文章页数】：109 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
LIST OF ABBREVIATIONS
CHAPTER 1 INTRODUCTION
1.1 General Introduction
1.2 Review of literature
1.2.1 Rice:present status
1.2.2 Salinity stress effects on rice production
1.2.3 Salinity stress effects on rice plants
1.3 Salinity stress sensing in rice plant
1.4 Response of rice under salinity
1.4.1 Morpho-physiological response
1.4.2 Response at the biochemical level
1.4.3 Response at molecular level
1.5 Defense system of rice against salinity stress
1.6 Transcriptional regulation and gene expression of salinity tolerance
1.7 Molecular genetic mapping analysis of QTLs
1.8 Genetics and QTL mapping of salinity tolerance
CHAPTER 2 Mapping Quantitative Trait Loci Associated with Salinity Tolerance in RIL Population of Rice(Oryza sativa L.)
2.1 Introduction
2.2 Materials and methods
2.2.1 Plant materials and population development
2.2.2 Treatment of salt stress
2.2.3 Phenotypic evaluation of parents and progenies
2.2.4 Statistical analysis
2.2.5 Construction of linkage map and QTL analysis
2.3 Results
2.3.1 Evaluation of phenotypic characteristics under salt stress
2.3.2 Correlation analysis of traits
2.3.3 Identification of QTLs for seedling-stage salt tolerance traits
2.4 Discussion
2.5 Conclusion
CHAPTER 3 Comparative Transcriptome Profiling of93-11 and PA64s and Identification of Candidate Genes Involved in Salinity Tolerance
3.1 Introduction
3.2 Materials and methods
3.2.1 Plant growth conditions and salinity stress treatment
3.2.2 Phenotypic evaluation of seedlings under salt stress treatment
3.2.3 RNA extraction and c DNA library preparation and sequencing
3.2.4 Data filtering and assembly
3.2.5 Analysis and functional annotation of DEGs
3.2.6 Preparation of RNA
3.2.7 Synthesis of c DNA
3.3 Results
3.3.1 PA64s seedlings are more tolerant to salinity than93-11 seedlings
3.3.2 m RNA sequencing and functional annotation
3.3.3 Expression profiling analysis in the PA64s and93-11 under salinity
3.3.4 Gene ontology(GO)enrichment classification of the DEGs
3.3.5 Identification of differential transcription factor(TFs)
3.3.6 KEGG pathway enrichment analysis of the DEGs
3.3.7 Identification of candidate genes within the stable QTLs via RNA-seq
3.4 Discussion
3.5 Conclusion
CHAPTER 4 Genetic Dissection of Major Quantitative Trait Locus q SL7 for Shoot Length under Salinity Stress in Rice(Oryza sativa L.)
4.1 Introduction
4.2 Materials and methods
4.2.1 Plant materials and population development
4.2.2 Growing conditions
4.2.3 Phenotypic evaluation of93-11,CSSL-q SL7 and mapping populations
4.2.4 Na+/K+ion measurement
4.2.5 Photosynthetic pigment content measurement
4.2.6 Determination of soluble sugar
4.2.7 Determination of proline
4.2.8 DNA isolation and PCR analysis
4.2.9 Statistical analysis of data
4.3 Result
4.3.1 CSSL-q SL7 shows more salt tolerant than93-11
4.3.2 Photosynthetic capacity in93-11 and CSSL-qSL7
4.3.3 Accumulation of soluble sugar and proline in93-11 and CSSL-q SL7
4.3.4 Fine mapping of qSL7
4.4 Discussion
4.5 Conclusion
CHAPTER 5 Major findings and future perspectives
5.1 Major findings
5.2 Future perspectives
REFERENCES
APPENDIX
LIST OF PUBLICATIONS
ACKNOWLEDGEMENT
AUTHOR'S RESUME
【参考文献】：
期刊论文
[1]水稻耐盐基因定位与克隆及品种耐盐性分子标记辅助选择改良研究进展[J]. 井文,章文华. 中国水稻科学. 2017(02)
[2]Selection of Rice Genotypes for Salinity Tolerance Through Morpho-Biochemical Assessment[J]. Md. Nasim ALI,Bhaswati GHOSH,Saikat GANTAIT,Somsubhra CHAKRABORTY. Rice Science. 2014(05)
[3]Dissection of genetic overlap of salt tolerance QTLs at the seedling and tillering stages using backcross introgression lines in rice[J]. ZANG JinPing 1 ,SUN Yong 1 ,WANG Yun 1 ,YANG Jing 1 ,LI Fang 1 ,ZHOU YongLi 1 ,ZHU LingHua 1 , Reys JESSICA2,Fotokian MOHAMMADHOSEIN 2,XU JianLong 1&LI ZhiKang 1,2 1Institute of Crop Sciences/National Key Facility for Crop Gene Resources&Genetic Improvement,Chinese Academy of Agricul- tural Sciences,Beijing 100081,China; 2International Rice Research Institute,DAPO Box 7777,Metro Manila,Philippines. Science in China（Series C:Life Sciences）. 2008(07)
[4]高质量植物基因组DNA的分离[J]. 罗志勇,周钢,陈湘晖,陆秋恒,胡维新. 湖南医科大学学报. 2001(02)
本文编号：3620363
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