新疆核桃微生物菌肥菌种组合筛选

发布时间：2018-01-06 07:26

本文关键词：新疆核桃微生物菌肥菌种组合筛选　出处：《新疆农业大学学报》2016年04期 　论文类型：期刊论文

【摘要】：为获得适合新疆自然条件下的核桃专用微生物菌肥菌种组合,从已获得的新疆本地核桃根际土壤中筛选出的解磷菌13株(解有机磷较强的6株:p-1、p-2、p-4、p-5、p-6、p-7;解无机磷较强的7株:p1、p2、p3、p4、p5、p6、p7)、解钾菌13株(k1、k2、k3、k5、k6、k7、k8、k9、k10、k11、k12、k13、k14)优势功能菌株为材料,进行生物拮抗测试,再根据不同功能分类(解有机磷、解无机磷、解钾)进行复合,研究其在混菌发酵条件下的解磷解钾功能表达,其中p-4+p2+k8组合与p-4+p5+k14组合解无机磷能力最强,解磷量达到300μg/mL;解有机磷最强的组合p-7+p1+k8组合与p-6+p7+k11组合,两菌种组合能力表达稳定且达到40μg/mL;解钾能力最强的组合为p-4+p1+k5组合、p-6+p4+k5组合、p-7+p7+k11组合,最终获得最佳菌种组合:p-7+p7+k11。
[Abstract]:In order to obtain a special microbial fertilizer combination of walnut suitable for natural conditions in Xinjiang, 13 strains of phosphorus releasing bacteria (6 strains with strong organophosphorus) were screened from the soil of walnut rhizosphere in Xinjiang. P-2n, p-4, p-5, p-6, p-7; The 7 strains with strong inorganic phosphorus, 1: p1, p2, p3, p4, p5, p6, P7, and 13 strains of k1k1, k2, k2, k3, k5, k6, k7, k8, k9k10, respectively. The dominant functional strains of k11k12k13k14 were used as materials for bioantagonism test, and then combined according to different functional classification (organic phosphorus, inorganic phosphorus, potassium). The functional expression of phosphorolytic potassium in mixed fermentation was studied. The combination of p-4p2k8 and p-4p5k14 had the strongest ability to hydrolyze inorganic phosphorus, and the amount of phosphorus was 300 渭 g / mL. The combination of p-7p1k8 and p-6p7k11, the strongest combination of organophosphorus, showed stable expression and reached 40 渭 g / mL. The best combination was p-4p1k5, p-6p4k5, p7p7k11, and the best strain combination: p-7p7k11 was obtained.
【作者单位】：新疆农业大学林业研究所;
【基金】：国家林业公益性行业项目(201304701) 国家自然科学基金项目(31360194)
【分类号】：S144;S664.1
【正文快照】： (Institute of Forestry,Xinjiang Agricultural University,Urumqi 830000,China)核桃(Juglans regia)在新疆的种植历史悠久。新疆核桃产业的发展和传统施肥方式的盛行使化肥的使用量激增,而普通化肥专用化程度低,在生产过程中易偏施,多施不但提高了成本,还会引起核桃营养失

【相似文献】