拟除虫菊酯对斑马鱼氧化代谢相关基因表达影响

发布时间：2018-01-12 02:20

本文关键词：拟除虫菊酯对斑马鱼氧化代谢相关基因表达影响　出处：《浙江工业大学学报》2016年03期 　论文类型：期刊论文

【摘要】：研究选取两种典型的拟除虫菊酯类农药——氯菊酯(PM,Ⅰ型)和高效氯氟氰菊酯(LCT,Ⅱ型),测定了药物暴露后斑马鱼肾脏中氧化代谢相关的三大类核受体基因的mRNA表达水平:芳香烃受体(AhR)、过氧化物增殖物酶体激活酶受体(PPAR)和孕烷X受体(PXR).结果表明:LCT暴露可导致AhR2,PPARα和PXR上调,PM暴露则导致AhR1a和PXR表达的上调;两种农药暴露均导致AhR1b下调;LCT在高质量浓度下对基因的影响更强,PM在环境暴露质量浓度下可产生一定效应.研究表明了LCT和PM对水生生态系统均存在生态风险,进一步揭示了拟除虫菊酯类农药的毒性作用机制,为其生态安全及环境健康风险评估提供理论依据.
[Abstract]:Study on the selection of two kinds of pyrethroid pesticides -- typical permethrin (PM, 1) and cyhalothrin (LCT II), after drug exposure in zebrafish kidney oxidative metabolism related to three kinds of nuclear receptor gene expression level of mRNA was determined: the aryl hydrocarbon receptor (AhR), peroxidase the proliferation of enzyme activation enzyme receptor (PPAR) and pregnane X receptor (PXR). The results showed that LCT exposure could lead to AhR2, PPAR alpha and PXR increase, PM exposure leads to the expression of AhR1a and PXR increases; two kinds of pesticide exposure resulted in down-regulation of AhR1b; LCT in high concentration of gene the more PM in environmental exposure can produce certain effect concentration. The results show that LCT and PM are the ecological risk to the aquatic ecosystem, further reveals the mechanism of toxicity of pyrethroid pesticides, and provide a theoretical basis for the ecological safety and environmental health risk assessment.

【作者单位】：浙江工业大学环境学院;
【基金】：国家自然科学基金重点项目(21337005);国家自然科学基金资助项目(21377119)
【分类号】：X592
【正文快照】： 拟除虫菊酯类农药具有广谱、低残留、高效、中等毒性和能生物降解等特性,已经成为替代高毒有机磷杀虫剂农药的第二大杀虫剂[1-2].到目前为止,全球销售额上亿美元的菊酯类杀虫剂占全球市场比例20%,使用面积占整个杀虫剂的25%[3].这些拟除虫菊酯类农药虽然对哺乳动物是低毒的,但

【参考文献】