微生物肥料高效解磷菌筛选及解磷机理探究

发布时间：2018-01-15 18:00

本文关键词：微生物肥料高效解磷菌筛选及解磷机理探究　出处：《江苏农业科学》2016年12期 　论文类型：期刊论文

【摘要】：芽孢杆菌已被广泛应用于微生物肥料的生产。以巨大芽孢杆菌11433作为出发菌株,对其进行紫外诱变,根据平板解磷圈直径与菌落直径比值进行初筛,再根据解磷能力进行复筛,并最终获得一株高效解磷菌。该菌株的摇瓶试验结果表明,发酵3 d后,pH值从7.00降到了4.03,其解磷含量可达到46.79μg/m L,较出发菌株11433的产量提高了115.62%,且其遗传稳定性良好。以得到的高产菌11433-D作为研究对象,对其解磷机理进行分析。发现有机酸的产生促进了解磷量的提高且使发酵液pH值降低。气相色谱质谱和高效液相色谱检测发现,巨大芽孢杆菌发酵液中的主要代谢产物为葡萄糖酸、乙酸、丙酸。
[Abstract]:Bacillus has been widely used in the production of microbial fertilizer. Bacillus megaterium 11433 was used as the starting strain for UV mutagenesis and screening was carried out according to the ratio of the diameter of the plate to the diameter of the colony. The strain was screened again according to its ability to dissolve phosphorus, and a high efficient strain was obtained. The results of shaking flask test showed that the pH value of the strain decreased from 7.00 to 4.03 after 3 days of fermentation. The content of phosphorus was 46.79 渭 g / mL, which was 115.62% higher than that of the original strain 11433. And its genetic stability was good. The high-yielding strain 11433-D was used as the research object. It was found that the production of organic acids promoted the increase of the amount of phosphorus and decreased the pH value of fermentation broth. Gas chromatography-mass spectrometry and high performance liquid chromatography were found. The main metabolites in fermentation broth of Bacillus megaterium are gluconic acid, acetic acid and propionic acid.
【作者单位】：天津科技大学天津市工业微生物重点实验室/工业发酵微生物教育部重点实验室;天津北洋百川生物技术有限公司;天津市现代职业技术学院;
【基金】：天津市科技计划(编号:14ZCZDTG0025)
【分类号】：S144;S182
【正文快照】： 磷在农业中起到重要作用,土壤有效磷的缺乏导致植物减产[1-2]。磷肥的施用能够促进各种代谢正常进行,植物生长发育良好,同时可提高植物的抗寒性和抗旱性。肥料中70%以上的水溶性磷与土壤中的Ca2+、Fe3+、Al3+等结合,转化为难溶性磷酸盐,很难被植物吸收利用[3]。然而巨大芽孢杆

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