石柱黄连根腐病根际土壤细菌微生态研究

发布时间：2018-01-25 20:47

本文关键词： 黄连根腐病土壤酶活性土壤养分高通量测序细菌　出处：《中国中药杂志》2017年07期 　论文类型：期刊论文

【摘要】：该研究应用Illumina Hiseq 2500高通量测序平台,分析了未种植黄连土壤、黄连健株及病株根际土壤的细菌种群丰富度及多样性变化,并结合土壤养分及酶活变化检测,对黄连根腐病产生的机制进行了深入探讨。高通量测序显示,栽培黄连造成了土壤细菌种群的丰富度的显著降低(P0.05)和细菌种群多样性的降低;罹患根腐病的黄连植株根际土壤细菌的种群丰富度要显著低于未种植和健康黄连土(P0.05),种群多样性要显著低于未种植黄连土样(P0.05),但与健株土样之间差异不显著。对土壤的养分及酶活测定结果表明,栽培黄连造成了土壤pH、有效磷、脲酶活性的显著降低,蔗糖酶活性的显著升高(P0.05);根腐病土样中有机碳的含量、碱解氮、过氧化氢酶和脲酶活性显著低于健康土样,速效磷和速效钾含量显著高于健康土样(P0.05)。综合分析表明,栽培黄连造成了土壤中细菌种群丰富度和多样性的降低;病株土样中,细菌种群丰富度的显著降低和种群多样性的降低,有可能是导致黄连根腐病产生的一个重要原因,此外,土壤有机碳和过氧化氢酶活性的显著减低,也许是导致黄连根腐病发生的一个诱因。
[Abstract]:In this study, Illumina Hiseq 2500 high-throughput sequencing platform was used to analyze the variation of bacterial population richness and diversity in the rhizosphere soil of non-cultivated Coptis chinensis, Rhizosphere of Coptis chinensis and diseased plants. The mechanism of root rot of Coptis chinensis was studied with soil nutrient and enzyme activity detection. High throughput sequencing showed that the root rot of Coptis chinensis was caused by Rhizoma Coptidis. Cultivated Rhizoma Coptidis caused a significant decrease in soil bacterial population richness (P0.05) and bacterial population diversity. The population richness of rhizosphere bacteria in rhizosphere soil of Coptis chinensis infected with root rot was significantly lower than that of non-cultivated and healthy Coptis chinensis P0.05N, and the population diversity was significantly lower than that of non-cultivated soil samples of Coptis chinensis (P0.05). But there was no significant difference between soil samples and healthy soil samples. The results of soil nutrient and enzyme activity test showed that the soil pH, available phosphorus and urease activity of cultivated Coptis chinensis were significantly decreased. The activity of sucrase increased significantly (P 0.05). The contents of organic carbon, alkali-hydrolyzed nitrogen, catalase and urease in root rot soil samples were significantly lower than those in healthy soil samples, and the contents of available phosphorus and available potassium were significantly higher than that of healthy soil samples P0.050.The comprehensive analysis showed that the organic carbon content in soil samples was significantly higher than that in healthy soil samples. Cultivation of Coptis chinensis resulted in the decrease of bacterial population richness and diversity in soil. The significant decrease of bacterial population richness and the decrease of population diversity in soil samples of diseased plants may be an important cause of root rot of Coptis chinensis. A significant decrease in soil organic carbon and catalase activity may be a contributing factor to root rot of Coptis chinensis.
【作者单位】：重庆市中药研究院;重庆市中药良种选育与评价工程技术研究中心;中国中医科学院中药资源中心重庆分中心;
【基金】：国家“十二五”科技支撑计划项目(2011BAI13B02-1) 国家科技基础性工作专项(2015FY11500-120) 重庆市平台与基地建设项目(cstc2014ptjd10001) 重庆市基本科研业务项目(2015cstc-jbky-01904)
【分类号】：S154.3;S435.672
【正文快照】： 2.重庆市中药良种选育与评价工程技术研究中心,重庆400065;3.中国中医科学院中药资源中心重庆分中心,重庆400065)黄连Coptis chinensis Franchet为毛茛科多年生草本植物,以根茎入药,药材名味连、鸡瓜连,为2015年版《中国药典》[1]一部收录,是我国常用大宗中药材。黄连主要分布

【相似文献】