豆科作物间作燕麦对土壤固氮微生物丰度和群落结构的影响

发布时间：2018-01-27 04:09

本文关键词： 豆禾间作 nif H基因荧光定量PCR 高通量测序群落组成　出处：《应用生态学报》2017年03期 　论文类型：期刊论文

【摘要】：利用荧光定量PCR和高通量测序的研究方法,比较了3种种植模式(燕麦单作,O;大豆/燕麦,OSO;绿豆/燕麦,OMO)对燕麦土壤固氮微生物数量和群落组成的影响.结果表明:与大豆和绿豆间作显著改变了燕麦土壤的理化性质.燕麦土壤固氮微生物nif H基因拷贝数为每克干土1.75×10~(10)~7.37×10~(10),拔节期和成熟期OSO和OMO中nif H基因拷贝数分别是O中的2.18、2.64和1.92、2.57倍,且各处理成熟期nif H基因拷贝数显著低于拔节期.样品稀释性曲线和覆盖度结果表明,各样品nif H基因测序结果可靠.与绿豆间作显著提高了燕麦土壤nif H基因的多样性.各样品固氮微生物属水平上优势类群均为Azohydromonas、固氮菌属、慢生根瘤菌属、Skermanella和在属水平上无法归类的固氮微生物,但各优势类群相对丰度存在差异.样品OTU的venn分布和主成分分析显示,拔节期和成熟期nif H基因群落结构存在差异,两个生育时期OSO和OMO具有更相似的nif H基因群落结构.表明与大豆和绿豆间作可显著提高燕麦土壤固氮微生物的数量,并影响固氮微生物的群落组成.
[Abstract]:Fluorescence quantitative PCR and high-throughput sequencing were used to compare three cropping patterns (Oat monoculture). Soybean / oatmeal Oso; Mung beans / oats. OMOO). The results showed that intercropping with soybean and mung bean significantly changed the physical and chemical properties of oat soil. Nitrogen fixation microorganism nif in oat soil. The copy number of H gene is 1.75 脳 10 ~ (1) / g dry soil. 10 (7. 37 脳 10 ~ (10)). The copy numbers of nif H gene in OSO and OMO at jointing stage and mature stage were 2.18 ~ 2.64 and 1.92 ~ 2.57 times of those in O, respectively. The copy number of nif H gene was significantly lower than that at jointing stage. The dilution curve and coverage of the sample showed that the number of copies of nif H gene was significantly lower than that at jointing stage. The results of nif H gene sequencing in each sample were reliable. The nif in oat soil was significantly improved by intercropping with mung bean. The diversity of H gene. The dominant group of nitrogen-fixing microorganism in all samples was Azohydromonas. Azotobacter, Rhizobium, Skermanella and nitrogen-fixing microbes that cannot be classified at the generic level. The venn distribution and principal component analysis of OTU showed that there were differences in the community structure of nif H gene between jointing stage and mature stage. OSO and OMO had more similar nif H gene community structure in the two growing periods, indicating that intercropping with soybean and mung bean could significantly increase the amount of nitrogen-fixing microorganisms in oat soil. The community composition of nitrogen-fixing microorganisms was also affected.
【作者单位】：中国农业大学农学院;中国农业科学院作物科学研究所/农业部作物生理生态重点实验室;吉林省白城市农业科学院;
【基金】：国家现代农业产业技术体系建设专项(CARS-08-B-1) 公益性行业(农业)科研专项(201503121-11,201503120)资助~~
【分类号】：S154.3;S344.2
【正文快照】： 3吉林省白城市农业科学院,吉林白城137000)本研究由国家现代农业产业技术体系建设专项(CARS-08-B-1)和公益性行业(农业)科研专项(201503121-11,201503120)资助This study wassupported by the China Agriculture Research System Project(CARS-08-B-1)and the Special Fund fo

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