小菜蛾COX7C基因的克隆与表达分析

发布时间：2018-01-30 18:04

本文关键词： 小菜蛾细胞色素C氧化酶抗药性 qPCR　出处：《福建农业学报》2017年04期 　论文类型：期刊论文

【摘要】：细胞色素C氧化酶(Cytochrome C oxidase,COX)是真核细胞线粒体内膜和需氧细菌细胞膜呼吸电子传递链的终端氧化酶,与ATP的合成密切相关,COX7C基因属于细胞色素C氧化酶核基因编码的一个亚基。本研究克隆了小菜蛾COX7C基因,测序后获得1条全长为213bp的PxCOX7C基因开放阅读框序列,进化树分析表明COX7C在同一目昆虫中也不是完全保守的。荧光定量PCR分析COX7C在小菜蛾毒死蜱品系(CRS)、氟虫腈品系(FRS)和敏感品系(SS)3龄幼虫中的表达水平,结果表明COX7C在3个品系中均有表达,且在FRS中的表达量显著高于SS,说明其可能与小菜蛾对氟虫腈解毒代谢相关。本研究对小菜蛾COX7C进行了初步的探索研究,为今后对COX7C进一步的功能研究奠定基础。
[Abstract]:Cytochrome C oxidase (Cytochrome C oxidase) is the terminal oxidase of respiratory electron transfer chain between mitochondrial intima of eukaryotic cells and aerobic bacteria. COX7C gene is a subunit of cytochrome C oxidase nuclear gene coding for ATP synthesis. The COX7C gene of Plutella xylostella was cloned in this study. After sequencing, an open reading frame sequence of 213bp PxCOX7C gene was obtained. Phylogenetic tree analysis showed that COX7C was not completely conserved in the same order insects. Fluorescence quantitative PCR was used to analyze COX7C in chlorpyrifos strains of Plutella xylostella. The expression level of COX7C in the 3rd instar larvae of FRS and the sensitive strain was higher than that of SS. The results showed that COX7C was expressed in all of the three strains, and the expression level in FRS was significantly higher than that in SS. The results suggested that it might be related to the detoxification of fluronitrile by Plutella xylostella. In this study, a preliminary study on the COX7C of Plutella xylostella was carried out, which laid a foundation for the further study of the function of COX7C in the future.
【作者单位】：福建农林大学应用生态研究所;福建农林大学生命科学学院;农业部闽台作物有害生物综合治理重点实验室;闽台特色作物病虫生态防控协同创新中心;
【基金】：国家自然科学基金项目(31230061、31320103922)
【分类号】：S433.4
【正文快照】： 小菜蛾Plutella xylostella L.属鳞翅目昆虫,主要取食十字花科植物,迁飞性强,对十字花科蔬菜生产造成重大影响[1-2]。据不完全统计,小菜蛾每年可造成约40亿~50亿美元的经济损失[3]。目前小菜蛾已对95种杀虫剂产生了抗性(ArthropodPesticide Resistance Database),包括生物农药

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