ABP9过表达转基因玉米在大田中的抗旱表现

发布时间：2018-01-31 20:37

  本文关键词： ABP9基因 转基因玉米 抗旱性 田间表型 营养生长和生殖生长阶段 籽粒产量　出处：《中国农业科学院》2016年博士论文　论文类型：学位论文

【摘要】：干旱在中国是严重限制植物生产力的主要环境因素,往往给农业造成巨大的经济损失。随着全球气候的变化,世界各地的农业生产面临着来自频繁极端天气条件的严重威胁。开发植物新品种,可以缓解环境压力对维持产量稳定的负面影响,是维持和增加农业生产的关键。玉米(Zea mays L.)是中国和世界的一种主要作物。干旱胁迫对其产量和产量的稳定性有很大的影响。提高玉米抗旱性已成为私人和公共部门的最优先考虑的问题之一。前期我们从玉米中克隆了转录因子ABP9基因,其过量表达可提高转基因拟南芥对包括干旱在内的多种逆境的抗性。为研究该基因在玉米中的功能,我们创制了过量表达ABP9基因的玉米。在本研究中,我们评估了不同启动子驱动ABP9表达的四种建构即Pabp9-ABP9.1,Pubi-ABP9.1,Pubi-ABP9.2和Pubi-ABP9-3xFLAG的转基因玉米植株在营养生长和生殖生长阶段大田干旱下以及正常灌溉下的田间表现(2015),并鉴定了多个表现强抗旱性的转基因事件。在营养生长期施加的干旱下,Pabp9-ABP9.1转基因事件201、206、212和214,Pubi-ABP9.1转基因事件611,612,616和617,Pubi-ABP9.2转基因事件707、714和717,以及Pubi-ABP9-3xFLAG转基因事件809和815与非转基因植株相比具有较高的叶绿素荧光,植被覆盖指数提高,雌雄开花间隔期缩短,穗粒数、百粒重、平均穗籽粒产量增加。生殖生长期施加的干旱下,在Pabp9-ABP9.1转基因事件201、202、204和214,Pubi-ABP9.1转基因事件617和618,Pubi-ABP9.2转基因事件707和714,以及Pubi-ABP9-3xFLAG转基因事件808和817中也观察到了同样的表型。在正常灌溉条件下,Pabp9-ABP9.1转基因事件201、210、212和214,Pubi-ABP9.1转基因事件611、617、612和618,Pubi-ABP9.2转基因事件702、707、714和717,Pubi-ABP9-3xFLAG转基因事件803、807、808和817与非转基因植株相比也表现出改善的性状,如高叶绿素荧光和植被覆盖指数,短雌雄开花间隔期,穗粒数、百粒重、平均穗籽粒产量提高。总之,本研究中鉴定的玉米转基因事件为进一步分析玉米中ABP9基因作用的分子机制及培育耐旱生物技术产品提供了宝贵资源。
[Abstract]:Drought is a major environmental factor that severely limits plant productivity in China, often causing huge economic losses to agriculture. Agricultural production around the world faces serious threats from frequent extreme weather conditions. The development of new plant varieties can alleviate the negative impact of environmental pressure on maintaining yield stability. Is the key to maintaining and increasing agricultural production. Zea mays L. Drought stress is a major crop in China and the world. Drought stress has a great impact on yield and yield stability. Improving drought resistance of maize has become one of the top priorities in the private and public sectors. The transcription factor ABP9 gene was cloned in maize. Overexpression can enhance the resistance of transgenic Arabidopsis thaliana to various stresses including drought. In order to study the function of this gene in maize we have created a maize with overexpression of ABP9 gene. We evaluated four constructs of ABP9 expression driven by different promoters, namely, Pabp9-ABP9.1 / Pubi-ABP9.1. Field performance of transgenic Maize plants of Pubi-ABP9.2 and Pubi-ABP9-3xFLAG under Field drought and normal Irrigation during vegetative and Reproductive growth. (2015). The transgenic events of Pabp9-ABP9.1 in vegetative growth period were identified, and 201,206,212 and 214 transgenic events of Pabp9-ABP9.1 were identified. Pubi-ABP9.1 transgene events 611,612,616 and 617 Pubi-ABP9.2 transgenic events 707,714 and 717. The Pubi-ABP9-3xFLAG transgenic events 809 and 815 had higher chlorophyll fluorescence, higher vegetation cover index and shorter flowering interval than those of non-transgenic plants. The number of grains per panicle, 100-grain weight and average grain yield per panicle increased. Under drought during reproductive growth, Pabp9-ABP9.1 transgenic events 201,202,204 and 214were observed. Pubi-ABP9.1 transgene events 617 and 618 Pubi-ABP9.2 transgenic events 707 and 714. The same phenotype was observed in Pubi-ABP9-3xFLAG transgene events 808 and 817 under normal irrigation conditions. Pabp9-ABP9.1 transgene events 201,210,212 and 214m Pubi-ABP9.1 transgenic events 611,617,612 and 618. Pubi-ABP9.2 transgenic event 702,707,714 and 717 Pubi-ABP9-3xFLAG transgenic event 803,807. Compared with the non-transgenic plants, 808 and 817 also showed improved characters, such as high chlorophyll fluorescence and vegetation cover index, short flowering interval between male and female, number of grains per ear and 100 kernel weight. In conclusion, the transgenic events identified in this study provided valuable resources for further analysis of the molecular mechanism of ABP9 gene function in maize and the cultivation of drought-tolerant biotechnology products.
【学位授予单位】：中国农业科学院
【学位级别】：博士
【学位授予年份】：2016
【分类号】：S513;S423
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本文编号：1479945