代森锰锌类农药对生姜种植地土壤酶活性及微生物群落结构的影响

发布时间：2018-02-02 14:46

本文关键词： 代森锰锌土壤酶微生物群落和结构　出处：《生态环境学报》2016年09期 　论文类型：期刊论文

【摘要】：研究了代森锰锌类农药克菌宝(15%)、深蓝(60%)和活根菌灭(80%)对生姜(Zingiber Officinale Roscoe)种植地土壤酶(脲酶、脱氢酶、磷酸酶和蛋白酶)活性和土壤微生物群落结构的影响,以期为评价代森锰锌的合理使用及环境修复提供一定的理论参考依据。结果表明,施用克菌宝的农田土壤在生姜旺盛生长期酸性磷酸酶活性提高了2.3%;深蓝能显著抑制旺盛生长期土壤酸性磷酸酶和蛋白酶活性(P0.05),抑制率分别为24.2%和40.0%,但这两种酶的活性在生姜收获期均有所恢复;活根菌灭在收获期可显著降低土壤酸性磷酸酶和脲酶活性(P0.05),土壤酶活性的抑制率与一定范围内(0.96 g·m~(-2))的农药施用量呈正相关,但当施用量超过一定阈值(0.72 g·m~(-2))后抑制率降低。3种农药对土壤酶活性抑制作用的强弱顺序为深蓝活根菌灭克菌宝;DGGE图谱的土壤微生物丰富度和Shannon-Wiener指数分析结果表明,土壤微生物的丰富度和多样性高低顺序为克菌宝深蓝活根菌灭,这与相应农药的土壤酶活性高低情况不一致,说明土壤细菌群落结构与土壤酶活性不是单一的线性关系。细菌16S r RNA基因的聚类分析表明细菌群落结构受到农药的影响,并与其施用量呈正相关。
[Abstract]:The mancozeb zinc insecticide was studied in this paper. The soil enzymes (urease, dehydrogenase) of Zingiber Officinale Roscoe were studied. The effects of phosphatase and protease) activity and the structure of soil microbial community are expected to provide a theoretical basis for evaluating the rational use of mancozeb and environmental remediation. The activity of acid phosphatase increased by 2.3in the vigorous growing period of ginger. Deep blue could significantly inhibit the activity of acid phosphatase and protease P0.05N in the soil during the vigorous growing period, and the inhibition rates were 24.2% and 40.0%, respectively. However, the activity of these two enzymes recovered in the harvest period of ginger. Rhizobium eradication significantly decreased soil acid phosphatase and urease activity (P0.05). The inhibition rate of soil enzyme activity was positively correlated with the amount of pesticide applied within a certain range of 0.96 g 路m ~ (-1) ~ (2). However, when the dosage exceeded a certain threshold value of 0.72 g / m ~ (-2), the inhibition rate decreased by .3 kinds of pesticides on soil enzyme activity. The results of soil microbial richness and Shannon-Wiener index analysis of DGGE map showed that the order of soil microbial richness and diversity was Keferbur deep blue living rhizobia. This was not consistent with the soil enzyme activity of the corresponding pesticides. The cluster analysis of 16s r RNA gene showed that the bacterial community structure was affected by pesticide. And positive correlation with its dosage.
【作者单位】：中国科学院水利部成都山地灾害与环境研究所;四川省烟草公司广元分公司;成都德通环境工程有限公司;中国科学院近代物理研究所;
【基金】：国家自然科学基金项目(41571315) 中国科学院仪器设备功能开发技术创新项目(Y6K2200200);中国科学院院地合作项目(Y406060YDO)
【分类号】：S154;S482.2
【正文快照】： 引用格式:李霞,张小平,喻晓,张丹,李战鲁,刘子明,王镧,李欣.2016.代森锰锌类农药对生姜种植地土壤酶活性及微生物群落结构的影响[J].生态环境学报,25(9):1569-1574.LI Xia,ZHANG Xiaoping,YU Xiao,ZHANG Dan,LI Zhanlu,LIU Zhiming,WANG Lan,LI Xin.2016.Effect of mancozeb o

【相似文献】