土壤悬液培养法研究长期施肥下花生根际解磷菌溶磷特性

发布时间：2018-02-07 11:54

本文关键词： 有机肥与无机肥配施土壤悬液培养法解磷微生物有效磷 pH　出处：《土壤学报》2017年01期 　论文类型：期刊论文

【摘要】：基于长期野外定位试验和室内土壤悬液培养,研究长期不同有机与无机肥料配施(纯化肥(NPK)、化肥与厩肥配施(NPKM)和化肥与稻草秸秆配施(NPKS))下,花生根际土壤解磷菌对Ca_3(PO_4)_2(Ca-P)、FePO_4(Fe-P)和AlPO_4(Al-P)的溶解特性。结果表明:有机无机肥配施促进了解磷菌的繁殖,在Ca-P和Fe-P固体NBRIP(国际植物研究所磷酸盐生长培养基)培养基中,NPKM处理可培养解磷菌密度分别为6.15和5.80 log(cfu g-1 dry soil),高于其他处理。在分别以Ca-P、Fe-P和Al-P为唯一磷源的NBRIP液体培养基中添加土壤悬液培养9 d发现,NPKM处理对Fe-P和Al-P的最高溶磷量分别为221.8 mg kg~(-1)和205.5 mg kg~(-1);NPKS处理对Ca-P的溶解有明显的优势。相比于单一菌株,解磷菌溶磷能力无绝对优势,但更能反映田间复杂条件下实际溶磷效果。通过土壤悬液培养法,从微生物群体角度发现:长期无机肥和厩肥配施更能促进花生根际解磷菌的繁殖以及对无机磷的溶解,从而改善土壤缺磷状况,提高花生生物量和产量。
[Abstract]:Based on the long-term field experiment and indoor soil suspension culture, the authors studied the long-term application of different organic and inorganic fertilizers (NPKN, NPKM) and NPKSs, respectively. The dissolving characteristics of Phosphorus bacteria in rhizosphere soil of Peanut on the basis of the dissolving characteristics of Cash2Ca-P0 (FePO4FeFe-P) and AlPO4AL-P). The results showed that the combination of organic and inorganic fertilizers promoted the propagation of Phosphorus solvans. In Ca-P and Fe-P solid NBRIP medium, the densities of Phosphorus decomposed bacteria were 6.15 and 5.80 log(cfu g -1 dry soiline, respectively, which were higher than those of other treatments. In the NBRIP solution with Ca-Pu Fe-P and Al-P as the sole phosphorus source, respectively, the densities of Phosphorus were 6.15 and 5.80 log(cfu / g ~ (-1), respectively. It was found that the highest phosphorus solubilization of Fe-P and Al-P were 221.8 mg / kg ~ (-1) and 205.5 mg / kg ~ (-1) respectively, and the solubilization of Ca-P was obviously superior to that of a single strain. There is no absolute superiority in phosphorus solubilizing ability of phosphorus solubilizing bacteria, but it can reflect the actual phosphorus dissolving effect under complex field conditions. From the point of view of microbial population, it was found that long-term application of inorganic fertilizer and barnyard manure could promote the propagation and dissolution of inorganic phosphorus in rhizosphere of peanut, thus improving the soil phosphorus deficiency and increasing the biomass and yield of peanut.
【作者单位】：中国科学院南京土壤研究所;中国科学院大学;中国科学院红壤生态实验站;
【基金】：中国科学院战略先导专项(XDB15030200) 江苏省自然科学青年基金项目(BK20141048) 国家自然科学基金项目(41471237)资助~~
【分类号】：S154.39;S565.2

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