一株具紫色土亲和性解钾菌的筛选及促生效应

发布时间：2018-02-09 07:03

本文关键词： 紫色土解钾菌环状芽孢杆菌钾素营养　出处：《西南大学学报(自然科学版)》2016年05期 　论文类型：期刊论文

【摘要】：解钾菌能改善土壤钾素有效性.为筛选具紫色土适应能力的土著解钾菌应用于专用微生物肥料,以实现替代钾肥的不当使用和提高钾素的利用率.本研究从重庆市北碚区紫色土中筛选出1株解钾菌(XD-K-2).结合菌体及菌落形态学特征、生理生化试验结果,以及基于16SrDNA的系统发育分析,该菌被鉴定为环状芽孢杆菌(Bacillus circulans sp.),并命名为B.circulans XD-K-2.测定其对钾长石的解钾能力,XD-K-2解钾量达到12.8 mg/L,与不接种对照相比,可溶性钾含量增加124%.小白菜促生试验表明,接种XD-K-2能显著促进小白菜的生长,在提高小白菜株高、总根长、地上部鲜/干质量等植物营养指标和紫色土可溶性钾素养分方面达到了施用钾肥的效果.综上,菌株XD-K-2是一株具紫色土高亲和性的解钾菌,可作为微生物钾肥研究候选菌株.
[Abstract]:Potassium bacteria can improve the availability of potassium in soil. In order to realize the improper use of alternative potassium fertilizer and to improve the utilization rate of potassium, a strain XD-K-2 was selected from purple soil of Beibei District of Chongqing City. The results of physiological and biochemical tests combined with the morphological characteristics of bacteria and colonies were analyzed. Based on the phylogenetic analysis of 16s rDNA, the strain was identified as Bacillus circulans sp. sp., and named B. circulans XD-K-2.The amount of potassium hydrolyzed by XD-K-2 to potassium feldspar reached 12.8 mg / L, compared with that of uninoculated control. The experiment of promoting growth of pakchoi showed that inoculation with XD-K-2 could significantly promote the growth of Chinese cabbage, and increase the plant height and total root length of pakchoi. The plant nutrition indexes such as shoot fresh / dry weight and soluble potassium quality of purple soil reached the effect of applying potassium fertilizer. In summary, strain XD-K-2 was a high affinity bacterium for potassium hydrolysis in purple soil, and could be used as a candidate strain for microbial potash fertilizer research.
【作者单位】：西南大学资源环境学院;农业部重庆紫色土生态环境重点野外科学观测试验站;
【基金】：国家自然科学基金项目(41573104)
【分类号】：S144

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