洞庭湖湿地木霉多样性及生防活性

发布时间：2018-02-10 22:32

本文关键词： 木霉洞庭湖湿地种类鉴定系统发育生防活性　出处：《微生物学通报》2017年10期 　论文类型：期刊论文

【摘要】：【目的】了解湖南省洞庭湖湿地木霉种类及分布,丰富我国的木霉种质资源,为功能菌株筛选应用奠定基础。【方法】利用ITS序列比对分析结合形态学特征对分离到的木霉菌株进行种类鉴定,构建系统发育进化树分析其亲缘关系。通过菌丝生长速率法测定菌株的平板抑菌能力,根据水解带大小检测菌株的水解酶活性,利用灰色关联度分析筛选综合生防效果较好的木霉菌株。【结果】从52个土样和18个水样中分离得到114株木霉菌株,经鉴定分属16个木霉种类:哈茨木霉、绿木霉、刺孢木霉、土星孢木霉、钩状木霉、拟康宁木霉、短密木霉、深绿木霉、猥木霉、毛细木霉(中国新记录种)、长枝木霉、卵孢木霉、侧耳木霉、加纳木霉、厚木霉及一个疑似新种;哈茨木霉为洞庭湖湿地中的优势种,占总菌株数量的19.30%;16种木霉在系统发育树中归于7个进化支:Harzianum Clade、Virens Clade、Longibrachiatum Clade、Lutea Clade、Viride Clade、Hamatum Clade、Unknown Clade。灰色关联度分析表明,菌株TW21990、QT22040和QT22094的灰色关联度较高,分别为0.849 5、0.798 6和0.732 6,综合生防性状较好。【结论】洞庭湖湿地木霉具有种类多样性和分布多样性,发现了一个中国新记录种毛细木霉和一个疑似新种,哈茨木霉TW21990、长枝木霉QT22040和卵孢木霉QT22094是潜在的优良生防菌株。
[Abstract]:[objective] to understand the species and distribution of Trichoderma in Dongting Lake, Hunan Province, and to enrich the germplasm resources of Trichoderma in China. [methods] ITS sequence alignment analysis combined with morphological characteristics was used to identify the species of Trichoderma strains. Phylogenetic tree was constructed to analyze the phylogenetic relationship. The bacteriostasis ability of the strain was determined by hyphal growth rate method, and the hydrolase activity of the strain was determined according to the size of the hydrolytic band. 114 Trichoderma strains were isolated from 52 soil samples and 18 water samples, belonging to 16 Trichoderma species: Trichoderma harzii, Trichoderma aeruginosa, Trichoderma spp. Trichoderma Saturn, Trichoderma hookiformis, Trichoderma kangonensis, Trichoderma brevis, Trichoderma aurolimus, Trichoderma obliquus, Trichoderma capillaris (newly recorded species in China, Trichoderma ovata, Trichoderma orientalis, Trichoderma, Trichoderma Ghana, Trichoderma thuringiensis and a new suspected species); Trichoderma harzii is the dominant species in the wetland of Dongting Lake, and 16 species of Trichoderma species, which account for the total number of strains, belong to 7 phylogenetic branches: 1: 1 Harzianum Cladea Clarens Cladea Clatea Cladea Clatea Cladea Clatea Cladea Clatea Cladee Clade.The grey correlation analysis shows that strain TW21990 QT22040 has a higher grey correlation degree with QT22094, and the grey correlation degree between TW21990 QT22040 and QT22094 is higher than that of TW21990 QT22040, and the grey correlation degree between TW21990 QT22040 and QT22094 is relatively high. It was 0.849 5% 0.798 6 and 0.732 6, respectively. The comprehensive biocontrol characters were better. [conclusion] Trichoderma martensii from Dongting Lake has species diversity and distribution diversity, and a new record species of Trichoderma capillaris and a suspected new species have been found. Trichoderma harziensis TW21990, Trichoderma QT22040 and Trichoderma ovalis QT22094 are potential biocontrol strains.
【作者单位】：山东省科学院生态研究所山东省应用微生物重点实验室;
【基金】：山东省重点研发计划项目(No.2015GNC113003) 公益性行业(农业)科研专项项目(No.201303025) 科技基础性工作专项项目(No.2014FY120900) 山东省农业重大应用技术创新项目 “十三五”国家重点研发项目(No.2017YFD0201102)~~
【分类号】：S476.1

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