小麦幼苗内生菌多样性的宏基因组分析

发布时间：2018-02-22 18:19

本文关键词： 小麦幼苗内生菌宏基因组多样性系统发育分析　出处：《植物病理学报》2017年03期 　论文类型：期刊论文

【摘要】：小麦是世界各地广泛种植的重要禾本科作物,在生长过程中与内生菌形成和谐共生的微生态体系,内生菌次生代谢产物具有抗病、防虫与促生等重要作用。宏基因组技术是研究环境微生物及内生菌多样性的重要手段。本研究首先富集纯化幼苗期小麦内生菌的宏基因组DNA,PCR扩增内生细菌的16S rDNA及内生真菌rDNA-ITS序列,构建了大肠杆菌克隆文库,选取阳性克隆测序。根据16S rDNA信息及系统进化树对内生细菌群初步分析,划分为9个分类操作单元(operational taxonomic unites,OTUs),主要包括γ-变形菌纲肠杆菌科(95.59%)、梭菌纲(1.47%)及不可培养细菌(2.94%),其中肠杆菌科菌群为优势菌。内生真菌rDNA-ITS克隆序列根据序列相似性和系统发育分析划归为13个OTUs,主要为不可培养真菌(37.50%)与子囊菌(62.50%),后者分属于半子囊菌纲(30.00%)、座囊菌纲(5.00%)与粪壳菌纲(27.50%)3个菌纲,其中不可培养真菌为优势菌。上述研究结果表明小麦幼苗内生菌遗传多样性分布较广,本研究为进一步挖掘利用小麦内生菌资源提供了基础信息。
[Abstract]:Wheat is an important gramineous crop widely planted all over the world. In the process of growth, wheat and endophytic bacteria form a harmonious and symbiotic microecological system. The secondary metabolites of endophytic bacteria are resistant to disease. Macrogenome technology is an important method to study the diversity of environmental microbes and endophytic bacteria. In this study, the macrogenomic DNA of wheat endophytic bacteria in seedling stage was first enriched and purified to amplify 16s rDNA of endophytic bacteria. RDNA-ITS sequence of endophytic fungi, Escherichia coli clone library was constructed, positive clones were selected and sequenced. According to 16s rDNA information and phylogenetic tree, the endophytic bacterial population was preliminarily analyzed. It is divided into 9 taxonomic taxonomic units, including 纬 -Proteus Enterobacteriaceae 95.59m, Clostridium 1.4775) and non-culturable bacteria 2.9410, in which Enterobacteriaceae is the dominant bacteria. The rDNA-ITS clone sequence of endophytic fungi is based on sequence similarity and similarity. Phylogenetic analysis was classified into 13 OTUs, mainly non-culturable fungi (37.50) and ascomycetes 62.50. The latter belong to the class of semiascomycetes 30.00m, cystacea 5.00) and fecal shell fungi 27.50). The results showed that the genetic diversity of endophytes in wheat seedlings was wide. This study provided basic information for the further exploitation of wheat endophyte resources.
【作者单位】：江苏省农业科学院植物保护研究所;
【基金】：国家公益性行业(农业)项目(201503112)
【分类号】：S182;S512.1

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