山东地区盐碱土花生种子际土壤微生物群落结构的研究

发布时间：2018-02-27 21:39

本文关键词： 花生盐碱土种子萌发种子际细菌多样性 lllumina Hiseq高通量测序　出处：《微生物学报》2017年04期 　论文类型：期刊论文

【摘要】：【目的】以不同含盐量的滨海盐土、内陆盐碱土和中等肥力非盐碱土壤为实验对象,探讨花生种子在吸水膨胀与萌发过程中,不同类型盐碱土对种子际土壤微生物多样性变化的影响。【方法】采集不同含盐量的滨海盐土、内陆盐碱土和中等肥力非盐碱土壤,通过对各样品中细菌的16S r RNA基因的V3-V4区进行PCR扩增,利用Illumina Hiseq高通量测序技术对12份V3-V4高变区PCR产物进行测序,并对测序数据进行生物信息学分析。【结果】(1)盐碱土壤的种子际细菌群落多样性高于非盐碱土壤,且以东营青坨滨海盐土种子际土壤细菌群落多样性较高。(2)不同类型土壤样本微生物群落结构在纲水平存在明显差异。4种土壤类型种子际土壤细菌共分属于6个菌纲,分别为Proteobacteria、Actinobacteria、Actinobacteria、Bacteroidetes、Acidobacteria和Firmicutes菌纲,并均以Proteobacteria和Actinobacteria菌纲为主要菌纲。全样本菌落结构分析结果表明,4种类型土壤中不同吸胀时间内种子际微生物菌落在门、属水平上的类型和丰度差异最为显著(P0.05)。(3)beta多样性分析和各样本遗传距离(phylogenetic distances)聚类树图分析表明,4个土壤类型的12个土壤样本种子际土壤中微生物群落均可聚为2大类。【结论】土壤含盐量越高其种子际土壤细菌群落多样性较高。不同类型土壤样本微生物群落结构在纲水平存在明显差异,以Proteobacteria和Actinobacteria菌纲为主要菌纲。种子吸胀萌发时间影响种子际微生物菌落在门、属水平上的类型和丰度,但对相同土壤类型样本间遗传距离无影响。
[Abstract]:[objective] to study the water absorption expansion and germination of peanut seeds in littoral saline soil, inland saline soil and medium fertility non-salinized soil with different salt contents. Effects of different types of saline-alkali soils on the variation of microbial diversity in interseed soil. [methods] Coastal saline soils with different salt contents, inland saline soils and moderately fertile non-salinized soils were collected. The V3-V4 region of 16s r RNA gene of bacteria was amplified by PCR, and 12 PCR products of V3-V4 hypervariable region were sequenced by Illumina Hiseq high-throughput sequencing technique. The sequence data were analyzed by bioinformatics. [results] the diversity of inter-seed bacterial community in saline-alkali soil was higher than that in non-saline-alkali soil. The microbial community structure of different soil samples in Qingtuo littoral salt soil was significantly different at the class level. 4 kinds of soil types belong to 6 classes of microbes, and the soil microbial community diversity of Qingtuo littoral saline soil was higher than that of Dongying Qingtuo littoral saline soil. The soil microbial community structure of different types of soil samples was obviously different at the class level. Proteobacteriae, Actinobacteriae, Actinobacteriae, BacteroidetesAcidobacteria and Firmicutes, with Proteobacteria and Actinobacteria as the main class respectively. The analysis of genetic distance and phylogenetic distances-cluster tree map showed that the microbial communities in 12 soil samples of 4 soil types could be clustered into 2 large soils. The results showed that the diversity of microbial communities in 12 soil samples of 4 soil types was significantly different from that of P0.05 and P0.05. The genetic distance of each sample was analyzed by phylogenetic distances-cluster tree map. [conclusion] the higher the salt content in soil, the higher the diversity of soil bacterial community in seed soil. Taking Proteobacteria and Actinobacteria as the main class, the time of seed bloating and germination affected the type and abundance of inter-seed microorganism at the level of phylum, but had no effect on the genetic distance between samples of the same soil type.
【作者单位】：山东省花生研究所;沈阳农业大学农学院;河北农业大学植物保护学院;
【基金】：“十三五”国家科技支撑计划(2014BAD11B04-03) 山东省现代农业产业技术体系创新团队岗位专家(花生)(SDAIT-04-06) 山东省农业重大应用技术创新课题(2014) 山东省自主创新专项基金(2014ZZCX07401-12)~~
【分类号】：S565.2;S154.3

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