无机磷溶解菌RW8的筛

发布时间：2018-02-28 10:43

本文关键词： 溶磷菌白三叶阴沟肠杆菌有机酸　出处：《草业学报》2017年05期 　论文类型：期刊论文

【摘要】：从白三叶根际分离筛选9株溶磷微生物,并对挑选菌株进行形态、细胞以及种属鉴定,并分析其对白三叶的促生效果。通过分析溶磷圈与溶磷菌落直径的大小与比值获得溶磷量与持续溶磷能力较强的RW8菌株。通过形态与扫描电镜观察发现RW8是具有鞭毛的短杆菌,其菌落形态表面光滑,不透明;将RW8 16SrDNA序列比对NCBI数据库发现其与阴沟肠杆菌属(Enterobacter sp.)的同源性高达99.5%,Biolog鉴定其与阴沟肠杆菌(Enterobacter cloacae ss dissolvens)的相似性为0.610。RW8溶磷量达424.85μg/mL,溶磷能力可能与RW8的产酸性能(12.30μg/mL)密切相关。RW8对白三叶根系与幼苗的生物产量的分配有显著影响,接种RW8后能抑制白三叶根系伸长,根系鲜重与干重有下降趋势,但差异不显著;而幼苗的生物产量(干重与鲜重)均显著高于对照(P0.05),并且该现象与植物生长激素IAA无关,其具体的作用机制还有待于进一步研究。
[Abstract]:Isolation of 9 strains of phosphate solubilizing microorganisms from white clover rhizosphere, and morphology of the selected strains, cells and species identification and analysis of the growth promoting effect of white clover. Through the analysis of soluble phosphorus and dissolved phosphorus ratio of ring size and colony diameter obtained dissolved phosphorus content and phosphorus solubilizing ability of the continuous RW8 by strain. The morphology and scanning electron microscope observation indicated that RW8 is Brevibacterium have flagella, the colony morphology of the surface is smooth, opaque; RW8 16SrDNA sequences of NCBI database and it is found that it belongs to Enterobacter cloacae (Enterobacter sp.) the homology was 99.5%, and the Biolog identification of Enterobacter cloacae (Enterobacter cloacae SS dissolvens) similarity phosphorus content of up to 424.85 g/mL 0.610.RW8 solution, phosphate solubilizing capacity may produce acid performance with RW8 (12.30 g/mL) significantly affected the distribution of.RW8 is closely related to white clover root and seedling biomass, after inoculation with RW8 can White clover inhibited root elongation, root fresh weight and dry weight decreased, but the difference was not significant; while the seedling biomass (dry weight and fresh weight) were significantly higher than those of control (P0.05), and this phenomenon and the plant growth hormone IAA is independent of the specific mechanism needs further study.

【作者单位】：贵州省草业研究所;贵州省生物技术研究所;
【基金】：贵州省农科院专项基金(黔农科院院专项[2013]03) 贵州省科技支撑计划(黔科合支撑[2016]2504号) 农科院自主创新科研专项(黔农科院自主创新科研专项字[2014]010号)资助
【分类号】：S182;S541.2

【相似文献】