川东低山丘陵区农业旅游活动对土壤微生物群落结构的影响

发布时间：2018-03-01 07:44

本文关键词： 川东低山丘陵区农业旅游土壤微生物群落　出处：《土壤通报》2017年01期 　论文类型：期刊论文

【摘要】：开展农业旅游对土壤生态环境影响的专题研究,可为休闲农业旅游区的合理开发和有效保护提供理论基础,为评估未来农业旅游活动对土壤生态的影响提供科学依据。本研究以重庆市铜梁区黄桷门奇彩梦园和北碚区金果园游步道两侧的土壤样区为研究对象,通过现场调查、采样以及磷脂脂肪酸谱图分析(PLFAs),系统研究并比较了土壤微生物受影响的距离、主要微生物群落组成及其受冲击程度。结果显示:微生物群落结构受冲击范围在4 m以内(P0.05),微生物生物量在土壤中的分布随着离游径的距离变小有明显下降趋势;在受农业旅游冲击的范围内细菌,革兰氏阳性细菌,革兰氏阴性菌,真菌的生物量随着离游径的距离变小有明显下降趋势,放线菌在部分样区表现出该规律,原生动物无此规律;对所测PLFAs数据进行多样性和冲击指数分析后得出假单胞菌(16∶0),好氧细菌G+(19∶0 cyclo w8c,15∶0 iso)等受影响程度较大,节杆菌(17∶0),真菌(18∶3 w6c(6,9,12))等受影响程度较小,受冲击程度大小与该微生物在生物总量中所占的比重呈正相关。综上,通过对两园区微生物群落进行评价分析发现调查样区土壤生态系统的稳定性已受到冲击。
[Abstract]:A special study on the impact of agricultural tourism on the soil ecological environment can provide a theoretical basis for the rational development and effective protection of recreational agricultural tourist areas. In order to provide scientific basis for evaluating the impact of agricultural tourism activities on soil ecology, this study took the soil sample areas on both sides of Huangjuaomen Qicaimeng Garden and Beibei Golden Orchard Walk Road in Tongliang District, Chongqing City, as research objects, and investigated on the spot. The sampling and phospholipid fatty acid spectrum analysis were used to study and compare the influence distance of soil microorganism. The results showed that the structure of microbial community was affected within 4 m (P 0.05), and the distribution of microbial biomass in soil tended to decrease with the distance from the path to the soil. The biomass of Gram-positive bacteria, Gram-negative bacteria, Gram-negative bacteria and fungi decreased with the distance from the path, and actinomycetes showed this rule in some sample areas. On the basis of analysis of diversity and impact index of PLFAs data, it was found that Pseudomonas sp. 16: 0, aerobic bacteria G: 19: 0 cyclo w8cn 15: 0 iso. et al were affected to a greater extent, while Arthrobacter sp. 17: 0 and fungi 183: 3 w6c691212) were less affected. The degree of impact was positively correlated with the proportion of the microorganism in the total biological amount. In summary, the stability of the soil ecosystem in the sample area was investigated by the evaluation and analysis of the microbial communities in the two parks.
【作者单位】：陕西省土地工程建设集团;国土资源部退化及未利用土地整治工程重点实验室;西南大学资源环境学院;
【基金】：国家“乡村土地流转与资源整合关键技术与示范”课题(2013BAJ11B02) 国家科技支撑计划(2014BAL01B03)资助
【分类号】：S154.3

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