广西富硒区土壤耐硒菌株的分离及鉴定

发布时间：2018-03-06 14:26

本文选题：富硒土壤　切入点：耐硒　出处：《西南农业学报》2017年10期 　论文类型：期刊论文

【摘要】：【目的】从广西富硒区土壤中分离出多株耐硒菌株,为土壤硒资源开发利用提供参考。【方法】在广西主要富硒区永福、巴马、玉林寒山、桂平、藤县等地采集田间土样,利用稀释平板法并通过加硒培养对耐硒微生物进行分离、筛选。【结果】筛选得到8株耐硒能力较强的菌株,其在固体培养基中对硒的耐受浓度均在10 000μg/m L以上。8株耐硒菌株中,YLB1-33耐硒能力最强,其在含硒量为29000μg/m L的固体培养基中仍能微弱生长。经16S rDNA序列分析和系统发育分析,鉴定结果表明,YLB1-6为蜡质芽孢杆菌、BMB2-1和TXB1-8为短小芽孢杆菌、GPB2-5为苏云金芽孢杆菌、YLB1-26和YLB1-33为地衣芽孢杆菌、YLB1-2和YFB1-8为粘质沙雷氏菌。【结论】耐硒菌株的发现对广西土壤硒资源利用、富硒农产品开发具有潜在的应用价值。
[Abstract]:[objective] to isolate many strains of selenium-tolerant strains from the soils of Se-rich areas of Guangxi, and to provide a reference for the exploitation and utilization of soil selenium resources. [methods] the soil samples were collected from Yongfu, Bama, Yulin, Hanshan, Guiping and Liangxian in the main selenium-rich areas of Guangxi. Selenium-tolerant microorganisms were isolated and screened by dilution plate method and selenium culture. [results] eight strains with strong selenium-tolerance were obtained. YLB1-33 had the strongest selenium tolerance in solid medium with selenium tolerance concentration above 10 000 渭 g / mL, and it could still grow weakly in solid medium containing 29000 渭 g / mL selenium. 16s rDNA sequence analysis and phylogenetic analysis were performed. The results showed that YLB1-6 was Bacillus cereus BMB2-1 and TXB1-8 was Bacillus pumilus GPB2-5, Bacillus thuringiensis YLB1-26 and YLB1-33 were Bacillus licheniformis YLB1-2 and YFB1-8 as clay Shareh bacteria. The development of selenium-rich agricultural products has potential application value.
【作者单位】：广西农业科学院农业资源与环境研究所;
【基金】：广西科学研究与技术开发计划项目(桂科合415104001-22) 广西科技计划项目(桂科攻1598006-5-13) 广西富硒特色作物试验站项目(桂TS201611) 广西农业科学院科技发展基金和科研业务费项目(桂农科2015JM23,2017JM01,2017JM03)
【分类号】：S154.3

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