牛粪堆肥中溶解性有机物对铜绿微囊藻生理生化及产毒特性的影响

发布时间：2018-03-07 19:05

本文选题：牛粪堆肥　切入点：溶解性有机物(DOM)　出处：《湖南农业大学学报(自然科学版)》2017年03期 　论文类型：期刊论文

【摘要】：提取牛粪堆肥中的溶解性有机物(dissolved organic matter,DOM),并将DOM转移至含有铜绿微囊藻Microcystis aeruginosa FACHB905细胞的培养液中,DOM含量以溶解性有机碳(DOC)质量浓度进行衡量,分别控制培养液中DOC的终质量浓度为0、15、30、60、90、120 mg/L(分别记为CK、T1、T2、T3、T4、T5)。通过测定藻细胞密度、色素含量和藻毒素含量,研究DOM对铜绿微囊藻生理生化及产毒特性的影响。结果表明:1)M.aeruginosa FACHB905受DOM影响,在处理后第2天,各DOM处理的细胞密度与CK相比均有所增加,T4处理的细胞密度(5.97×106个/m L)最大,比CK增加了21.67%,处理后第4天,T1、T2、T3处理均能够促进细胞生长,T2处理的细胞密度(9.23×10~6个/m L)最大,比CK增加了15.27%,而T4、T5处理抑制细胞生长,处理后第6天,仅T1及T2处理能够促进细胞生长,T1处理的细胞密度(13.34×10~6个/m L)最大,比CK增加了10.35%,而T3、T4、T5处理抑制细胞生长;2)堆肥来源DOM能够抑制藻毒素的合成,单位细胞藻毒素Microcystin LR含量在处理后第2天T5处理的最低,比CK下降了53.13%,而Dha7 Microcystin LR含量在处理后第4天T5处理的最低,与CK比较下降了78.51%;3)各DOM处理对单位细胞内Chl a和Carotenoid的合成没明显影响。
[Abstract]:Dissolved organic matter (dissolved organic matterus) was extracted from cow manure compost, and DOM was transferred to the culture medium containing Microcystis aeruginosa FACHB905 cells. The content of Dom was measured by the mass concentration of dissolved organic carbon (DOC). The final mass concentration of DOC in culture medium was controlled to be 0 1530g / L, 6090g / L, respectively. The effects of DOM on physiological, biochemical and toxic properties of microcystis aeruginosa were studied by determining the cell density, pigment content and phycotoxin content of microcystis aeruginosa, respectively. The results showed that DOM was affected by DOM, and the results showed that the microcystis aeruginosa FACHB905 was affected by DOM, and the cell density, pigment content and phycotoxin content of microcystis aerginosa were determined. On the second day after treatment, the cell density of each DOM treatment was 5.97 脳 106 / mL, which was higher than that of CK, and the cell density of T4 treatment was 5.97 脳 106 / mL. On the 4th day after treatment, the cell density of T 2 treatment increased by 9.23 脳 10 ~ 6 / mL, and increased 15.27% than CK, while T4 + T _ 5 inhibited cell growth, and on the 6th day after treatment, the cell density was increased by 9.23 脳 10 ~ 6 / mL. Only T 1 and T 2 treatments could promote cell growth. The cell density of T 1 treatment was 13.34 脳 10 ~ 6 / mL, which was 10.35% higher than CK, while T _ 3 T _ 4 T _ 4 T _ 5 treatment inhibited cell growth. DOM, a compost source, could inhibit the synthesis of phycotoxin. The content of Microcystin LR was the lowest in T5 treatment on the 2nd day after treatment, which was 53.13% lower than that of CK, while the lowest in T5 treated with Dha7 Microcystin on the 4th day after treatment. Compared with CK, there was no significant effect of DOM treatment on the synthesis of Chl a and Carotenoid in the unit cells.
【作者单位】：湖南农业大学资源环境学院;
【基金】：科技部水专项(2014ZX07206001 03)
【分类号】：S141.4

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