新型木霉氨基酸有机肥研制及其对番茄的促生效果

发布时间：2018-03-08 02:34

本文选题：贵州木霉　切入点：氨基酸　出处：《应用生态学报》2017年10期 　论文类型：期刊论文

【摘要】：为提高贵州木霉NJAU4742固体发酵产孢能力,本研究拟用稻草秸秆和氨基酸水解液为原料进行固体发酵获得高分生孢子含量的固体菌种,将该固体菌种添加到含有不同比例氨基酸水解液的有机肥中进行二次发酵研制新型生物有机肥,并利用盆栽试验研究该生物有机肥对番茄的促生作用.结果表明:在利用稀释30倍的氨基酸水解液浸泡秸秆过夜后调节秸秆为初始p H 3.5、75%含水量、30%玉米粉添加量的条件下,贵州木霉NJAU4742所产分生孢子数量高达2.40×10~(10)CFU·g~(-1).接种2%固体木霉菌种于含有20%氨基酸水解液的腐熟有机肥后,该处理中木霉活菌数和吲哚乙酸(IAA)含量均达到最高,分别为6.40×10~9CFU·g~(-1)和38.66 mg·kg~(-1),与对照相比分别增加了1142.30和1.42倍.盆栽试验表明,木霉氨基酸生物有机肥(AT)与氨基酸有机肥(AA)处理跟对照(CK)相比株高分别增加了98.8%和23.8%,茎粗分别增加了58.9%和10.3%,叶绿素、叶长、叶宽等指标也显著增加.利用稻草秸秆和氨基酸水解液进行浅盘固体发酵生产木霉分生孢子,克服了工厂化生产固体木霉菌种的瓶颈,以此制备的新型木霉氨基酸有机肥(AT)与等养分的氨基酸有机肥(AA)和对照(CK)处理相比,对番茄的促生效果显著,在集约化农业生产中具有良好的应用前景.
[Abstract]:In order to improve the spore production ability of Trichoderma Guizhou NJAU4742 solid fermentation was carried out with straw straw and amino acid hydrolysate as raw materials to obtain solid strains with high conidial content. The solid strain was added to organic fertilizer containing different proportion of amino acid hydrolysate for secondary fermentation to produce new biological organic fertilizer. Pot experiment was used to study the effect of bio-organic fertilizer on tomato growth. The results showed that under the condition of adjusting straw to initial pH 3.5% 75% water content and 30% corn flour after soaking straw overnight with 30 times of diluted amino acid hydrolysate, the effect of organic fertilizer on tomato growth was studied. The number of conidia produced by Trichoderma NJAU4742 in Guizhou was as high as 2.40 脳 10 ~ (10) NJAU4742. After inoculating 2% solid Trichoderma spp. After inoculation with mature organic fertilizer containing 20% amino acid hydrolysate, the viable number of Trichoderma and the content of Indole Acetate (IAA) reached the highest in the treatment. It was 6.40 脳 10 ~ (9 CFU 路g ~ (-1)) and 38.66 mg 路kg ~ (-1) ~ (-1), which was 1142.30 and 1.42 times higher than that of the control, respectively. The plant height, stem diameter, chlorophyll and leaf length were increased by 98.8% and 23.8g, 58.9% and 10.3, respectively, in the treatments of Trichoderma and Amino acid organic fertilizer (AAA) and CK, respectively, in comparison with the CK of the control group, the plant height was increased by 98.8% and 23.8.The stem diameter was increased by 58.9% and 10.3, respectively. Leaf width was also significantly increased. The shallow-dish solid fermentation of Trichoderma spore with straw straw and amino acid hydrolysate overcame the bottleneck of industrial production of solid Trichoderma species. Compared with Amino acid organic fertilizer (AAA) and CK), the new Trichoderma organic fertilizer (ATT) has a good effect on tomato growth and has a good application prospect in intensive agricultural production.
【作者单位】：南京农业大学江苏省固体有机废弃物资源化高技术研究重点实验室/江苏省有机固体废弃物资源化协同创新中心;
【基金】：国家重点基础研究发展规划项目(2015CB150506) 江苏省自然科学基金项目(BK20150059)资助~~
【分类号】：S641.2;TQ440.7

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