金佛山方竹根际土壤中优势AM真菌的鉴定

发布时间：2018-03-09 11:34

本文选题：金佛山方竹　切入点：AM真菌鉴定　出处：《竹子学报》2017年02期 　论文类型：期刊论文

【摘要】：从贵州省正安县、桐梓县和重庆市南川区的金佛山方竹林地采集方竹根系和根际土壤,采用碱解离-酸性品红染色法对根样进行染色,研究根系丛枝菌根侵染情况,利用湿筛倾注-蔗糖离心法从土样中分离AM真菌孢子,结合形态特征和18S r DNA AML1-AML2区序列的系统发育分析对AM真菌进行种属鉴定。结果显示:AM真菌能够侵染金佛山方竹根系,侵染率为25.27%;从土壤中分离得到6种优势AM真菌,其中4种通过形态特征和18S r DNA AML1-AML2区序列的系统发育分析,均能鉴定到种水平,分别为蜜色无梗囊霉Acaulospora mellea、珠状巨孢囊霉Gigaspora margarita、美丽盾巨孢囊霉Scutellospora calospora和孢璧两性球囊霉Ambispora leptotich,其余2种通过18S r DNA AML1-AML2区序列的系统发育分析鉴定为无梗囊霉属Acaulospora,结合形态特征鉴定为大型无梗囊霉Acaulospora colossica和疣状无梗囊霉Acaulospora tuberculata。
[Abstract]:Root system and rhizosphere soil were collected from Zhenan County, Tongzi County and Nanchuan District of Chongqing City. The root samples were stained with alkaline dissociation-acid fuchsin staining method to study the infection of arbuscular mycorrhizal mycorrhizae of root plexus. The spores of AM fungi were isolated from soil samples by wet sieve pouring and sucrose centrifugation. The species of AM fungi were identified by morphological characteristics and phylogenetic analysis of 18s r DNA AML1-AML2 region. The results showed that the species of AM fungi could infect the root system of Phyllostachys chinensis in Jinfushan. The infection rate was 25.27. Six dominant AM fungi were isolated from soil, 4 of which were identified by phylogenetic analysis of 18s r DNA AML1-AML2 region. The other two species were identified as Acaulospora by phylogenetic analysis of 18s r DNA AML1-AML2 region, respectively, including Acaulospora melleaa, Gigaspora margarita, Scutellospora calospora and Ambispora leptotich. respectively. The morphological characteristics were identified as Acaulospora colossica and Acaulospora tuberculata.
【作者单位】：贵州大学生命科学学院;
【基金】：国家科技支撑计划项目(2015BAD04B0204) 贵州省科学技术基金项目(黔科合J字[2009]2282号)
【分类号】：S714.3

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