皂角苷强化白腐真菌Phlebia brevispora降解林丹的研究

发布时间：2018-03-11 18:24

本文选题：皂角苷　切入点：林丹　出处：《西南农业学报》2017年05期 　论文类型：期刊论文

【摘要】：【目的】为了提高环境中持久性有机氯农药的生物降解效果。【方法】利用平衡振荡法研究了生物表面活性剂皂角苷对有机氯农药林丹的增溶作用,并通过液相培养法研究了皂角苷对白腐真菌Phlebia brevispora生长以及降解林丹的强化效果。【结果】皂角苷能大幅度增加林丹的溶解度,实验浓度范围内增溶倍数最高可达到7.4。皂角苷作为生长基质可促进P.brevispora的生长,提高其生物量,但浓度在达到1.0 g/L时,菌株生物量几乎不再继续增加。在0.05~1.0 g/L浓度范围内,皂角苷能显著提高菌株对溶液中林丹的降解效果(P0.05),且降解率随着皂角苷浓度的增加而增加,降解率最大可提高14.1%。添加0.5和1.0 g/L的皂角苷处理时,菌株胞内粗酶液对林丹的降解率显著高于不添加皂角苷的处理(P0.05)。皂角苷对林丹的增溶效果随着无机盐的加入而提高,20 mmol/L的Na Cl和Na_2SO_4使林丹的溶解度提高了1.17和1.26倍;而在含有皂角苷的降解体系中,Na Cl的添加进一步促进了林丹的生物降解效果,降解率提高了10%以上。【结论】本研究结果表明,利用皂角苷强化微生物降解去除环境中的有机污染物是可行的,具有一定的实际应用价值。
[Abstract]:[objective] to improve the biodegradation of persistent organochlorine pesticides in the environment. [methods] the solubilization of biosaponin on Lin Dan was studied by equilibrium oscillation method. The effects of saponins on the growth of white rot fungus Phlebia brevispora and the degradation of Lin Dan were studied by liquid phase culture. [results] saponin could significantly increase the solubility of Lin Dan. The solubilization ratio of P. brevispora was up to 7.4 in the range of experimental concentration. Saponin could promote the growth and increase the biomass of P.brevispora, but when the concentration reached 1.0 g / L, the biomass of P. brevispora was almost no longer increased, and in the range of 0.05g / L, the biomass of P. brevispora was almost no longer increased. Saponins could significantly improve the degradation of Lin Dan in the solution, and the degradation rate increased with the increase of saponin concentration, and the degradation rate was increased by 14.1g / L. when 0.5 and 1.0 g / L saponins were added, the degradation rate increased with the increase of the concentration of saponin. The degradation rate of Lin Dan by crude enzyme solution was significantly higher than that without saponin. The solubilization effect of saponins on Lin Dan increased by 1.17 and 1.26 times with the addition of inorganic salt, NaCl and Na_2SO_4 for 20 mmol/L. In the degradation system containing saponins, the biodegradation effect of Lin Dan was further promoted by the addition of sodium chloride, and the degradation rate was increased by more than 10%. [conclusion] the results of this study show that the biodegradation rate of Lin Dan is more than 10%. It is feasible to use saponin to enhance microbial degradation and removal of organic pollutants in the environment, which has certain practical application value.
【作者单位】：东北林业大学林学院;九州大学农学院;
【基金】：国家自然科学基金项目(41201307) 教育部留学回国人员科研启动基金([2013]693)
【分类号】：X172;X592

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