堆肥过程中纤维素酶活与纤维素降解相关研究

发布时间：2018-03-13 16:18

  本文选题：堆肥　切入点：CMC酶　出处：《东北农业大学学报》2016年06期 　论文类型：期刊论文

【摘要】：采用室内小型堆肥反应器堆肥,测定CMC酶活性和β-葡萄糖苷水解酶活性,测定堆肥物料中纤维素、半纤维素和木质素相对含量,利用高效液相法测定葡萄糖和纤维二糖。结果表明,堆肥过程中,堆体温度前3 d迅速上升至45℃,进入高温期,维持8 d后进入降温期,最后与环境温度变化一致;β-葡萄糖苷水解酶活性第4天达最大值(1.482μmol p-Nitr·g-1DW·min-1),并在降温腐熟期维持在0.429~0.533μmol p-Nitr·g-1DW·min-1范围内;CMC酶活性变化趋势与β-葡萄糖苷水解酶活性相似,并在第7天达最大值(47.67μg glucose·g-1DW·min-1);纤维素和半纤维素降解趋势相似,均在高温期降解明显;木质素相对含量总体变化较小,且在降温腐熟期小幅增加。结果显示,CMC酶活性与纤维素和半纤维素降解呈正相关。
[Abstract]:The enzyme activities of CMC and 尾 -glucoside hydrolase were determined by composting in a small composting reactor, and the relative contents of cellulose, hemicellulose and lignin in compost materials were determined. Glucose and cellobiose were determined by high performance liquid phase method. The results showed that during composting, the first 3 days of composting temperature increased rapidly to 45 鈩，

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