土壤细菌对土壤中绿僵菌孢子萌发的影响

发布时间：2018-03-18 17:35

本文选题：平沙绿僵菌　切入点：土壤细菌　出处：《中国生物防治学报》2017年06期 　论文类型：期刊论文

【摘要】：平沙绿僵菌Metarhizium pingshaense是一种潜力巨大的生防真菌,其能否在土壤中快速定殖是有效控制地下害虫的重要条件,而孢子萌发是定殖的第一步。本文研究了竹林覆盖地和非覆盖地2种土壤在灭菌和添加抗生素条件下对绿僵菌孢子萌发的影响,并利用16S rDNA基因序列测序方法进一步分析土壤细菌中影响平沙绿僵菌孢子萌发的细菌类群。结果表明,在覆盖地土壤中平沙绿僵菌孢子萌发率较高,与非覆盖地差异显著;土壤灭菌处理后,非覆盖地土壤中平沙绿僵菌孢子萌发率显著提高,与覆盖地孢子萌发率差异不显著。添加6种抗生素后,平沙绿僵菌孢子萌发率在非覆盖地土壤中差异显著,在覆盖地差异不显著。非覆盖地土壤中细菌总数及多样性均高于覆盖地土壤,其中酸杆菌门Acidobacteria、放线菌门Actinobacteria和芽单胞菌门Gemmatimonadetes显著高于覆盖地土壤。
[Abstract]:Metarhizium Metarhizium Metarhizium pingshaense is a biocontrol fungus with great potential, and its ability to colonize rapidly in soil is an important condition for effective control of underground pests. Spore germination was the first step of colonization. The effects of sterilizing and antibiotics addition on the germination of Metarhizium anisopliae spore were studied. The 16s rDNA gene sequence sequencing method was used to further analyze the bacterial groups of soil bacteria affecting the germination of Metarhizium pingshadii spores. The results showed that the germination rate of Metarhizium pingshaensis spore was higher in the covered soil, and the difference was significant between the soil and the non-covered soil. After soil sterilization, the germination rate of Metarhizium anisopliae in uncovered soil was significantly higher than that in mulched soil, but there was no significant difference between the germination rate of Metarhizium pingshaensis spore and that of mulch soil. After adding 6 kinds of antibiotics, the germination rate of Metarhizium pingshaensis was significantly different from that in uncovered soil. The total number and diversity of bacteria in uncovered soil were higher than those in mulched soil, among which acidobacterium, actinomycetes Actinobacteria and Gemmatimonadetes were significantly higher than those in mulched soil.
【作者单位】：中国林业科学研究院亚热带林业研究所;
【基金】：公益性行业(林业)科研专项(201304403) 国家重点研发计划项目(2016YFD0600903)
【分类号】：S476.12

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