2株溶磷、解钾与产IAA的内生真菌菌株的筛

发布时间：2018-03-23 04:04

本文选题：溶磷　切入点：解钾　出处：《中国土壤与肥料》2017年03期 　论文类型：期刊论文

【摘要】：从钾矿区优势蕨类植物-芒萁内生真菌中筛选出具溶磷、解钾、分泌吲哚乙酸(IAA)的功能菌株,并研究其对农作物的促生作用。采用菌株的形态学特性、培养特征及5.8S rDNA、18S rDNA ITS序列分析方法对菌株进行鉴定,并结合液体培养和固体培养方法初步测定菌株的溶磷、解钾能力,采用回接及盆栽试验研究它们对玉米幼苗的促生作用。从42株芒萁内生真菌中筛选得到2株具有高效溶磷、解钾、分泌IAA功能的内生真菌菌株(编号为MQ013和MQ039),经鉴定MQ013菌株为泡盛曲霉(Aspergillus awamori),MQ039菌株为黑曲霉(Aspergillus niger)。促生作用试验结果显示,MQ013、MQ039菌株能有效提高玉米植株体内叶绿素及磷、钾含量。MQ013、MQ039菌株具有一定的溶磷、解钾和分泌IAA活性,并对玉米幼苗的生长有明显的促进作用,该菌株在研制高效生物肥料接种剂方面可能具有较大潜力。
[Abstract]:The functional strains of phosphate soluble, potassium releasing and indoleacetic acid (IAA) secreting from endophytic fungi of Osmunda Osmunda, a dominant fern in potassium mining area, were screened and their promoting effects on crops were studied. The morphological characteristics of the strains were used. The strain was identified by 5.8S rDNA18s rDNA ITS sequence analysis, and the ability of dissolving phosphorus and potassium was preliminarily determined by liquid culture and solid culture. Two of the 42 endophytic fungi of Osmunda Osmunda were screened out of the 42 strains of endophytic fungi, 2 of which had high solubility in phosphorus and potassium, and the growth of maize seedlings was studied by means of back-to-back and potted experiments. The endophytic fungi strains secreting IAA function (MQ013 and MQ039) were identified as Aspergillus awamorius MQ039 and Aspergillus awamorius MQ039 as Aspergillus Niger. The potassium content. MQ013 / MQ039 strain has the activity of dissolving phosphorus, releasing potassium and secreting IAA, and it can promote the growth of maize seedling obviously. The strain may have great potential in developing high efficiency bio-fertilizer inoculum.
【作者单位】：九江学院药学与生命科学学院;
【基金】：国家自然科学基金项目(31360064;51264014) 2014年江西省普通本科大学生创新创业教育计划项目(赣财教指[2014]57号)
【分类号】：S144

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