铅锌尾矿区土壤有机菌肥改良后植物根际菌群的特性及多样性研究

发布时间：2018-03-23 19:07

本文选题：根际菌群　切入点：有机菌肥　出处：《中南林业科技大学学报》2017年06期

【摘要】：以辣椒的根际菌群为研究对象,通过纯培养的正交试验对比分析有机菌肥改良前后根际菌群去除铅锌能力及特性的差异,同时利用Illumina Miseq平台对根际菌群的16S rRNA基因V4区域进行测序,分析细菌群落结构及多样性的变化。结果表明:施用有机菌肥提高了可培养根际菌群去除铅、锌的能力,去除率增量最大分别可达40.2%、12.7%。同时,菌群去除铅的最佳培养条件发生了改变,铅锌浓度和培养基类型对铅去除率影响的显著性有所提高。有机菌肥对锌去除率的影响在试验分析中体现不明显。培养基种类是影响菌群去除铅锌的最主要因素,菌群中的原核生物较真核生物更具去除铅锌的优势。有机菌肥的应用对细菌群落结构产生了一定影响,微生物的相对丰度发生了改变,菌群多样性得到了丰富,但硫杆菌属Thiobacillus仍是绝对的优势菌。
[Abstract]:The rhizosphere microflora of capsicum was used as the research object. The differences of the ability and characteristics of rhizosphere microflora to remove lead and zinc before and after the improvement of organic fertilizer were compared and analyzed by orthogonal experiment of pure culture. At the same time, Illumina Miseq platform was used to sequence the V4 region of 16s rRNA gene in rhizosphere microflora, and the changes of bacterial community structure and diversity were analyzed. The results showed that the application of organic fertilizer improved the ability of culturable rhizosphere microflora to remove lead and zinc. The maximum increment of removal rate can reach 40.2% and 12.77.At the same time, the optimum culture conditions for the removal of lead by bacteria have been changed. The effects of the concentration of lead and zinc and the type of culture medium on the removal rate of lead and zinc were significantly increased. The effect of organic bacterial fertilizer on the removal rate of zinc was not obvious in the experiment. The type of culture medium was the most important factor affecting the removal of lead and zinc by microbial community. Compared with eukaryotes, prokaryotes have more advantages in removing lead and zinc. The application of organic fertilizer has a certain effect on the structure of bacterial communities, the relative abundance of microbes has changed, and the diversity of bacteria has been enriched. But Thiobacillus is still the dominant bacterium.
【作者单位】：中南林业科技大学环境科学与工程学院;
【基金】：国家“十二五”科技支撑计划项目(2012BAC09B03-4) 国家“十二五”科技惠民计划项目(2012GS430203) 湖南省环境科学与工程重点学科建设项目
【分类号】：S154.3

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