拮抗多种植物病原真菌Streptomyces triostinicus C2的分离与鉴定

发布时间：2018-03-23 19:48

本文选题：分离与鉴定　切入点：放线菌菌株C　出处：《生物技术通报》2016年07期

【摘要】：旨在分离筛选获得对植物病原真菌具有抑制作用的拮抗菌。以水稻纹枯病菌为指示菌,采用平板对峙培养法进行拮抗菌的分离筛选;根据菌体形态学观察、生理生化特征以及16S r DNA序列分析,对拮抗菌进行菌种鉴定。结果显示,从采集自云南、广东、安徽、湖北、江西等地的18个土样中分离筛选到一株对水稻纹枯病菌具有良好抑制效果的菌株C2,该菌株初步鉴定为Streptomyces triostinicus,并暂命名为链霉菌C2;进一步测定链霉菌C2的抗菌谱发现,它对水稻纹枯病菌、水稻稻瘟病菌、葡萄炭疽病菌、西瓜枯萎病菌和橘青霉等多种植物病原真菌均具有良好的抑制作用,其抑菌率分别为49.78%、56.62%、80.96%、18.59%和94.10%。链霉菌C2是一株植物病原真菌广谱拮抗菌。
[Abstract]:The aim of this study was to isolate and screen antagonistic fungi with inhibitory effect on plant pathogenic fungi. Using rice sheath blight bacteria as indicator, the antagonistic bacteria were isolated and screened by means of plate confrontation culture. Physiological and biochemical characteristics and 16s r DNA sequence analysis were used to identify the antagonistic bacteria. A strain C2 was isolated from 18 soil samples from Jiangxi and other places. The strain was identified as Streptomyces triostinicus and was tentatively named Streptomyces C2.The antimicrobial spectrum of Streptomyces C2 was further determined. It has a good inhibitory effect on rice sheath blight, rice blast fungus, grape anthracnose, watermelon wilt and orange penicillium. The inhibitory rate of Streptomyces C2 was 18.59% and 94.10%, respectively. Streptomyces C2 was a broad-spectrum antagonistic fungus.
【作者单位】：江西农业大学作物生理生态与遗传育种教育部重点实验室江西省作物生理生态与遗传育种重点实验室;江西农业大学生物科学与工程学院江西省农业微生物资源开发与利用工程实验室;
【基金】：国家科技支撑计划项目(2011BAD16B0402,2013BAD07B12) 江西省青年科学家培养对象计划项目(20142BCB23025) 国家级大学生创新创业训练计划项目(201410410008)
【分类号】：S476.1

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