养殖鱼塘底泥微生物抗生素耐药基因分布分析

发布时间：2018-03-28 00:32

  本文选题：养殖鱼塘　切入点：微生物　出处：《环境科学学报》2017年10期

【摘要】：针对水产养殖中抗生素滥用引起微生物耐药基因污染的现象,本实验以杭州市某水产养殖区底泥样品为研究对象,测定了底泥样品中抗生素含量,并从其中分离出74株可培养细菌.采用PCR方法对分离菌株中磺胺类、四环素类、喹诺酮类、氯霉素类抗性基因(antibiotic resistance genes,ARGs)和整合子基因进行了检测.结果表明,除tet A(四环素类)耐药基因未被检出外,其余3种耐药基因及整合子基因均被检出,其中qnr S(喹诺酮类)耐药基因的检出率最高,为50.00%.另外,利用16S r DNA序列分析技术将分离菌株鉴定为12个属、19个种,包括环境中多种常见土著细菌及部分致病菌,其中气单胞菌(Aeromonas spp.)数量最多(29株),占分离菌株的39.19%.掌握水产养殖区中ARGs的污染现状,对控制其传播、保障食品安全和保护微生态环境具有重要指导意义.
[Abstract]:In view of the phenomenon of microbial drug resistance gene contamination caused by antibiotic abuse in aquaculture, the content of antibiotics in sediment samples in a aquaculture area of Hangzhou was determined. 74 strains of culturable bacteria were isolated from them. Sulfonamides, tetracyclines, quinolones, chloramphenicol resistant genes and integron genes were detected by PCR. Except tet A (tetracycline) resistance gene was not detected, the other three drug resistance genes and integron genes were detected. The detection rate of qnr S (quinolones) resistance gene was the highest (50.00g). The isolated strains were identified into 12 genera and 19 species by 16s r DNA sequence analysis, including a variety of common indigenous bacteria and some pathogenic bacteria in the environment. Among them, Aeromonas spp.has the largest number of 29 strains, accounting for 39.19% of the isolated strains. It is of great significance to grasp the status of ARGs pollution in aquaculture area, to control its spread, to ensure food safety and to protect the microecological environment.
【作者单位】： 中国计量大学生命科学学院;浙江省海洋食品品质及危害物质控制技术重点实验室;
【基金】：国家自然科学基金(No.31601464) 浙江省自然科学基金项目(No.LQ15C200001)~~
【分类号】：X172;X714
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本文编号：1673979