NaCl胁迫对滨梅根际细菌群落多样性及优势菌群的影响

发布时间：2018-03-30 16:12

本文选题：滨梅　切入点：NaCl胁迫　出处：《南京林业大学学报(自然科学版)》2017年04期

【摘要】：【目的】研究盐胁迫下耐盐植物根际微生物群落变化情况。【方法】基于温室盆栽试验,结合植株生长测定、土壤理化性质分析、PCR-DGGE图谱技术、香农多样性分析、主成分分析等方法,研究NaCl(0、2.9、8.8 g/L)溶液胁迫下,耐盐果树滨梅根际和非根际土壤细菌多样性变化情况及变化规律,以及滨梅生长及土壤理化性质变化。【结果】盐胁迫60 d后,高浓度NaCl(8.8 g/L)溶液处理下滨梅根际土壤细菌16s rDNA序列变性梯度凝胶电泳(DGGE)图谱中的条带亮度、多样性指数、均匀度指数和丰富度指数最高,非根际土壤中的条带数明显减少,条带亮度变暗,丰富度指数最低。对DGGE谱带进行聚类分析发现,不同谱带间具有较高的相似性(62%),其中根际盐处理试验组和根际对照组可聚为一类,非根际盐处理试验组可聚为一类,非根际对照组单独聚为一类。选择9条主要优势条带克隆测序,其中细菌类群为变形菌门、拟杆菌门、酸杆菌门和放线菌门。这些优势菌中除放线菌Candidatus属外,其他均对植物生长具有促进作用。对主要优势菌的变性梯度凝胶电泳(DGGE)图谱进行主成分(PCA)分析发现,非根际细菌与根际细菌在第一主成分上完全分离,非根际细菌均聚为一类,而根际细菌则按亲缘关系聚类。【结论】在耐盐果树滨梅正常生长时,一定程度的盐胁迫不但未能对根际土壤微生物群落造成伤害,反而起到一定的促进作用,这种促进作用体现为植株根系对有益细菌生长的保护和促进,以及对群落多样性的维持,而非根际土壤细菌的生长和群落多样性则受到了较为严重的伤害。
[Abstract]:[objective] to study the changes of rhizosphere microbial communities of salt-tolerant plants under salt stress. [methods] based on greenhouse experiment, combined with plant growth determination, soil physical and chemical properties were analyzed by PCR-DGGE and Shannon diversity analysis. Principal component analysis (PCA) was used to study the variation and regularity of bacterial diversity in the rhizosphere and non-rhizosphere soil of salt-tolerant fruit trees under NaCl (2.9g / L) stress, and the changes of the growth and physical and chemical properties of the soil. [results] after 60 days of salt stress, the changes of bacterial diversity in the rhizosphere and non-rhizosphere soil of salt-tolerant fruit trees were studied. The bands brightness, diversity index, evenness index and richness index were the highest in the 16s rDNA sequence denaturing gradient gel electrophoresis (DGE) map of the rhizosphere soil bacteria treated with high concentration NaCl(8.8 g / L solution, and the number of bands in the non-rhizosphere soil decreased obviously. The results of cluster analysis of DGGE bands showed that there was a high similarity between different bands, among which the experimental group of rhizosphere salt treatment and the control group of rhizosphere could be clustered into one group. The non-rhizosphere salt treatment group could be divided into one group and the non-rhizosphere control group alone. Nine major dominant bands were selected for cloning and sequencing, in which the bacterial groups were Proteus and Bacteroides. Acidobacterium and actinomycetes. Except for actinomycetes Candidatus, other dominant bacteria can promote plant growth. Non-rhizosphere bacteria and rhizosphere bacteria were completely separated from rhizosphere bacteria on the first principal component. Non-rhizosphere bacteria were clustered into one group, while rhizosphere bacteria were clustered according to phylogenetic relationship. [conclusion] when salt-tolerant fruit trees grew normally, A certain degree of salt stress not only did not harm the rhizosphere soil microbial community, but also played a certain role in promoting the growth of beneficial bacteria, which was reflected in the protection and promotion of plant roots to the growth of beneficial bacteria. And the maintenance of community diversity, but the growth of bacteria and community diversity in non-rhizosphere soil were seriously damaged.
【作者单位】：江苏省·中国科学院植物研究所;
【基金】：国家海洋公益性行业科研专项项目(201505023) 江苏省农业“三新”工程项目(SXGC(2015)332) 江苏省木本油料高效栽培示范项目(LYSX[2015]14) 江苏省农业科技自主创新项目(CX(15)1026)
【分类号】：S154.3

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