基于LC-MS技术的代谢组学方法研究根肿菌对拟南芥代谢的影响

发布时间：2018-03-31 05:06

本文选题：根肿菌　切入点：拟南芥　出处：《华中农业大学学报》2017年05期

【摘要】：为探寻根肿菌侵染拟南芥后期寄主代谢的生物标志物,将罹患根肿病的油菜根切块进行组织培养,获得较为纯化的、单一的根肿菌接种体,与拟南芥种子在1/2MS培养基上共培养,利用液相色谱-质谱联用(LCMS)技术的非靶向代谢组学方法分析根肿菌侵染后期拟南芥根部代谢物差异。组织培养结果表明,根肿菌可以在愈伤组织中生长和繁殖;共培养结果显示45d后拟南芥侧根尖端肿大,显微观察显示根肿菌能完成侵染循环;代谢组数据PCA和PLS-DA分析结果显示试验组(接种根肿菌)和对照组(未接种根肿菌)明显分离;OPLSDA分析和t检验结果表明重要的差异代谢物包括油菜素内酯、类黄酮、木质素、萜类、甾体皂苷和磷脂等物质。提示这些物质可能在根肿菌侵染后期时拟南芥与根肿菌的相互作用中起重要作用。
[Abstract]:In order to explore the biomarkers of host metabolism in late stage of Arabidopsis thaliana infection by Rhizomegaly bacteria, the root of Brassica napus infected by root-swelling disease was cultured by tissue culture, and a single rhizomegaly inoculum was obtained by co-culture with Arabidopsis seeds on 1/2MS medium. The difference of metabolites in root of Arabidopsis thaliana in the later stage of infection was analyzed by non-target metabolomics method of liquid chromatography-mass spectrometry. The results of tissue culture showed that rhizomegaly bacteria could grow and reproduce in callus. The results of co-culture showed that the lateral root tip of Arabidopsis thaliana was enlarged after 45 days. Microscopic observation showed that rhizomegaly bacteria could complete the infection cycle. The results of PCA and PLS-DA analysis showed that the experimental group (inoculated with root-swelling bacteria) and the control group (without inoculation of root-swelling bacteria) were significantly separated by OPLSDA analysis and t-test results showed that important differential metabolites included rapesinolactone, flavonoid, lignin. Terpenoids steroidal saponins and phospholipids suggest that these substances may play an important role in the interaction between Arabidopsis thaliana and rhizomegaly in the late stage of rhizomegaly infection.
【作者单位】：华中农业大学植物科学技术学院/华中农业大学农业微生物学国家重点实验室/华中农业大学湖北省作物病害监测和安全控制重点实验室;
【基金】：现代农业产业技术体系建设专项资金(CARS-13)
【分类号】：S432.4

【相似文献】