2株耐热真菌的分离鉴定及其产酶活性研究

发布时间：2018-04-01 20:35

本文选题：耐热真菌　切入点：热气泉　出处：《新疆农业科学》2017年07期

【摘要】：【目的】对新疆玛纳斯火烧洼热气泉土壤样品中,分离出的2株耐热真菌MHS7和MHS9进行鉴定,并研究其产酶活性。【方法】利用平板稀释涂布法分离筛选耐热真菌,基于菌株形态学特征和r DNA ITS序列分析,对分离得到的耐热真菌菌株进行初步分类鉴定;分别测定各菌株产淀粉酶、蛋白酶、纤维素酶、脂肪酶、木聚糖酶和糖苷酶的能力。【结果】得到2株耐热真菌MHS7和MHS9,初步鉴定菌株MHS7属于小裸囊菌属(Gymnascella),菌株MHS9属于曲霉属(Aspergillus)。产酶活性检测表明,菌株MHS7和MHS9均能产淀粉酶、蛋白酶、纤维素酶和脂肪酶,其中,菌株MHS9产淀粉酶和蛋白酶的活性相对较高。【结论】耐热真菌MHS7和MHS9均能同时产四种酶,具有潜在的应用价值。
[Abstract]:[Objective] to Xinjiang Manasi fire hot air fountain in the soil samples wa 2 strains, MHS7 and MHS9 of thermotolerant fungi were identified, and study its enzyme activity. [method] screening of thermotolerant fungi by using dilution plate method, strain DNA morphological characteristics and R ITS sequence analysis based on preliminary classification and identification of heat resistant fungal strains isolated from different strains; Determination of amylase, cellulase, lipase and protease respectively, the ability of xylanase and glycosidase. [results] MHS7 and MHS9 2 strains of resistant fungi, preliminary identification of the strain MHS7 belong to small gymnoascus (Gymnascella), belongs to the genus Aspergillus strain MHS9 (Aspergillus). Show that detection of enzyme activity, strains MHS7 and MHS9 could produce amylase, protease, cellulase and lipase, among them, strain MHS9 producing amylase and protease activity is relatively high. [Conclusion] thermotolerant fungi MHS7 And MHS9 can produce four kinds of enzyme at the same time, which has potential application value.

【作者单位】：新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室;
【基金】：新疆维吾尔自治区自然科学基金项目“新疆玛纳斯热气泉高温菌分离鉴定及产酶研究”(2013211A044)~~
【分类号】：Q93

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