大兴安岭森林火烧后不同演替阶段土壤细菌多样性动态

发布时间：2018-04-02 13:04

本文选题：大兴安岭　切入点：森林演替　出处：《微生物学通报》2017年08期

【摘要】：【目的】研究我国最大的林区之一——大兴安岭森林火烧后不同演替阶段土壤细菌多样性动态,为天然林保护工程对于生物多样性的影响增添新的认识。【方法】以空间替代时间的方法分析大兴安岭森林演替对于土壤细菌多样性动态的影响。大兴安岭森林火烧后典型的自然演替序列为火烧迹地(LG-BA)、灌丛(SHR)、白桦林(BP)、白桦落叶松混交林(BP-LG)、落叶松林(LG-OM)。在演替序列的每个典型样地上采集0-10 cm土样,采用Illumina Mi Seq高通量测序技术测定土壤细菌群落组成及其多样性。【结果】细菌操作分类单位(OTU)数量从少到多的顺序为火烧迹地落叶松白桦混交林灌丛落叶松林白桦林。随着森林演替,多样性指数Simpson先增高后降低;Shannon指数先增加后减少再增加;OTU的丰度变化比较平缓,表明物种变化较小。在各演替阶段中,土壤细菌种类主要有变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)和浮霉菌门(Planctomycetes),4个门的种类含量随演替顺序都呈现先增加后减少的趋势。主成分分析表明不同演替阶段土壤细菌群落存在一定的差异性。冗余分析表明有机质(SOM)、全氮(TN)、全磷(TP)和p H对于土壤细菌群落变化有影响。【结论】随着森林演替,大兴安岭地区土壤细菌种类和生物多样性会发生变化,其变化与土壤化学成分和p H有关。
[Abstract]:[objective] to study the dynamics of soil bacterial diversity in different succession stages of Daxinganling forest, one of the largest forest regions in China. To add a new understanding of the impact of natural forest conservation engineering on biodiversity. [methods] the effects of forest succession on soil bacterial diversity dynamics in Daxinganling Mountains were analyzed by spatial substitution time. Forest Fire in Daxing'an Mountains. The typical natural succession sequence after burning was LG-BAG, SHRA, BPP, BP-LGN, LG-OM.The soil samples of 0-10 cm were collected from each typical sample of the succession sequence. Illumina Mi Seq high throughput sequencing technique was used to determine the composition and diversity of soil bacterial community. Forest. With the succession of forests, The diversity index (Simpson) increased at first, then decreased, then decreased, then increased, and then increased slowly, indicating that the variation of species was relatively small. The main species of soil bacteria were Proteobacteriae, Acidobacteriae, Actinobacteria, Actinobacteria and Planctomycetesus. The species contents of the four phylum increased first and then decreased with the succession sequence. The principal component analysis showed that the soil content increased first and then decreased in different succession stages. The results of redundancy analysis showed that organic matter (SOM), total nitrogen (TNN), total phosphorus (TP) and pH had influence on the change of soil bacterial community. [conclusion] with forest succession, soil bacterial community was changed. The species and biodiversity of soil bacteria in Daxing'anling region will change, which is related to soil chemical composition and pH.
【作者单位】：内蒙古农业大学沙漠治理学院;内蒙古大兴安岭林管局营林处;
【基金】：国家自然科学基金项目(No.41461106) 内蒙古农业大学引进人才科研启动项目(No.YJ2014-1)~~
【分类号】：S714.3

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