拮抗核盘菌Bt菌株的筛选及抑菌活性研究

发布时间：2018-04-04 18:09

  本文选题：核盘菌　切入点：Bt菌株　出处：《植物保护》2017年06期

【摘要】：利用平板对峙试验从170株Bt菌株中筛选获得12株对核盘菌Sclerotinia sclerotiorum有抑菌活性的菌株,对其中4株活性较强的菌株进行抗病相关基因分析,发现菌株中都含有几丁质酶基因,测定4AP1菌株发酵液的几丁质酶活力为672.4U/mL。通过对4AP1菌株pH和温度敏感性的研究发现,4AP1菌株最高可耐受55℃高温及pH 12缓冲液的处理,抑菌活性与对照无显著差异。且该菌株抑菌谱较广,除核盘菌外,对小麦赤霉病菌Fusarium graminearum等其他9种病原真菌均有抑制作用。本研究获得的4AP1菌株对核盘菌具有较强抑制作用,且耐热耐强碱环境,为构建杀虫抗病Bt工程菌提供了菌株资源。
[Abstract]:Twelve strains with antimicrobial activity against Sclerotinia sclerotiorum were obtained from 170 BT strains by plate confrontation test. Four of them with high activity were analyzed for disease resistance related genes. It was found that chitinase genes were found in all strains.The chitinase activity of fermentation broth of 4AP1 strain was 672.4 U / mL.Through the study of the pH and temperature sensitivity of 4AP1 strain, it was found that the highest tolerance to 55 鈩，

本文编号：1711091